2021-07-23 17:16:41 -04:00
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2021-07-30 13:56:09 -04:00
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2021-07-30 13:56:09 -04:00
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sodipodi:role="line"
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id="tspan283">30</tspan></text>
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</g>
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<g
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id="g287" />
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<g
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id="g289" />
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<g
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id="g291" />
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<g
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id="g293" />
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<g
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id="g295" />
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<g
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id="g297">
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<text
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transform="matrix(1,0,0,-1,48.82,6.8)"
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style="font-variant:normal;font-weight:normal;font-size:6px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text301"><tspan
|
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x="0 3.6659999 4.9980001 9.9960003 13.332 15 16.997999 21.996 23.327999 26.664"
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y="0"
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sodipodi:role="line"
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id="tspan299">Time (min)</tspan></text>
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</g>
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<g
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id="g303" />
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<g
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id="g305">
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<text
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transform="matrix(0,1,1,0,9.79,36.51)"
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style="font-variant:normal;font-weight:normal;font-size:6px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text309"><tspan
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|
x="0 4.0019999 5.3340001 8.6700001 10.338 11.67 15.006 16.674 20.01 21.677999 23.346001 26.681999 29.681999 33.018002 36.354 39.689999 41.358002 43.355999 46.692001 51.689999 55.026001 56.358002"
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y="0"
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sodipodi:role="line"
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id="tspan307">Biotin attached (nmol)</tspan></text>
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</g>
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<g
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id="g311" />
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<g
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id="g313" />
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<g
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id="g315" />
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<g
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id="g317" />
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<g
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id="g319" />
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<g
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id="g321" />
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<g
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id="g323" />
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<g
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id="g325">
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<path
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d="M 107.64,47.38 H 164.6 V 83.99 H 107.64 Z"
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style="fill:#ffffff;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="path327" />
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</g>
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<g
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id="g329" />
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<g
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id="g331" />
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<g
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id="g333" />
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<g
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id="g335">
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<text
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transform="matrix(1,0,0,-1,110.48,76.19)"
|
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style="font-variant:normal;font-weight:normal;font-size:6px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
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id="text339"><tspan
|
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|
|
x="0 4.0019999 7.3379998 8.6700001 10.158 13.494 16.997999 21.33 25.662001 29.664 33.167999 37.169998 38.501999 41.838001 43.506001 44.838001"
|
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|
y="0"
|
|
|
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|
|
sodipodi:role="line"
|
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id="tspan337">Sulfo−NHS−Biotin</tspan></text>
|
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<text
|
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|
transform="matrix(1,0,0,-1,110.48,69.71)"
|
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|
style="font-variant:normal;font-weight:normal;font-size:6px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
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id="text343"><tspan
|
|
|
|
|
|
x="0 4.3319998 7.6680002 11.004 14.004 17.34 20.676001 22.344 24.282 27.618 29.285999 30.618 33.953999 37.290001 38.958 40.956001 44.292 49.290001"
|
|
|
|
|
|
y="0"
|
|
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|
|
sodipodi:role="line"
|
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|
id="tspan341">Concentration (uM)</tspan></text>
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</g>
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<g
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id="g345" />
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<g
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id="g347" />
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<g
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id="g349" />
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<g
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id="g351" />
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<g
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id="g353">
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<path
|
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|
d="m 113.28,62.1 c 0,0.63 0.52,1.15 1.16,1.15 0.63,0 1.15,-0.52 1.15,-1.15 0,-0.64 -0.52,-1.16 -1.15,-1.16 -0.64,0 -1.16,0.52 -1.16,1.16"
|
|
|
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|
|
style="fill:#f8766d;fill-opacity:1;fill-rule:nonzero;stroke:#f8766d;stroke-width:0.71;stroke-linecap:round;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
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id="path355" />
|
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|
</g>
|
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<g
|
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id="g357" />
|
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<g
|
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id="g359">
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<path
|
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d="m 111.27,62.1 h 6.33"
|
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|
|
style="fill:none;stroke:#f8766d;stroke-width:0.43;stroke-linecap:butt;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
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id="path361" />
|
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</g>
|
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<g
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id="g363" />
|
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<g
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id="g365" />
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<g
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id="g367" />
|
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<g
|
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id="g369">
|
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|
<path
|
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|
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d="m 113.28,54.18 c 0,0.63 0.52,1.15 1.16,1.15 0.63,0 1.15,-0.52 1.15,-1.15 0,-0.64 -0.52,-1.16 -1.15,-1.16 -0.64,0 -1.16,0.52 -1.16,1.16"
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|
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style="fill:#00bfc4;fill-opacity:1;fill-rule:nonzero;stroke:#00bfc4;stroke-width:0.71;stroke-linecap:round;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
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id="path371" />
|
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|
</g>
|
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<g
|
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id="g373" />
|
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<g
|
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id="g375">
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<path
|
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d="m 111.27,54.18 h 6.33"
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|
|
style="fill:none;stroke:#00bfc4;stroke-width:0.43;stroke-linecap:butt;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
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id="path377" />
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|
</g>
|
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<g
|
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id="g379" />
|
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<g
|
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id="g381" />
|
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<g
|
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id="g383" />
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<g
|
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id="g385">
|
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<text
|
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|
transform="matrix(1,0,0,-1,121.39,60.3)"
|
|
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|
|
style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text389"><tspan
|
|
|
|
|
|
x="0 2.78"
|
|
|
|
|
|
y="0"
|
|
|
|
|
|
sodipodi:role="line"
|
|
|
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|
|
id="tspan387">75</tspan></text>
|
|
|
|
|
|
</g>
|
|
|
|
|
|
<g
|
|
|
|
|
|
id="g391" />
|
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<g
|
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|
id="g393" />
|
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<g
|
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|
id="g395" />
|
|
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<g
|
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|
|
id="g397" />
|
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<g
|
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|
|
id="g399">
|
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|
<text
|
|
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|
|
transform="matrix(1,0,0,-1,121.39,52.38)"
|
|
|
|
|
|
style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
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|
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|
|
id="text403"><tspan
|
|
|
|
|
|
x="0 2.78 5.5599999"
|
|
|
|
|
|
y="0"
|
|
|
|
|
|
sodipodi:role="line"
|
|
|
|
|
|
id="tspan401">125</tspan></text>
|
|
|
|
|
|
</g>
|
|
|
|
|
|
<g
|
|
|
|
|
|
id="g405" />
|
|
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|
|
|
<g
|
|
|
|
|
|
id="g407" />
|
|
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|
|
<g
|
|
|
|
|
|
id="g409" />
|
|
|
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|
|
<g
|
|
|
|
|
|
id="g411" />
|
|
|
|
|
|
<g
|
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|
|
id="g413" />
|
|
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|
<g
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id="g415" />
|
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<g
|
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id="g417" />
|
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<g
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id="g419" />
|
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<g
|
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id="g421" />
|
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<g
|
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id="g423" />
|
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<g
|
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id="g425" />
|
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<g
|
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|
id="g427" />
|
|
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<g
|
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id="g429" />
|
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|
</g>
|
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|
<g
|
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|
|
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id="g633"
|
|
|
|
|
|
inkscape:label="fraction_attached"
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|
|
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transform="matrix(1.3333333,0,0,-1.3333333,250.97336,164.53334)">
|
|
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|
<g
|
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|
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id="g635" />
|
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<g
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id="g637" />
|
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<g
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id="g639" />
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<g
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id="g641" />
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<g
|
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id="g643" />
|
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<g
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id="g645" />
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<g
|
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|
id="g647" />
|
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<g
|
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id="g649" />
|
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<g
|
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|
id="g651" />
|
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<g
|
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|
id="g653" />
|
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<g
|
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|
id="g655" />
|
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<g
|
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id="g657" />
|
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<g
|
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|
id="g659" />
|
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<g
|
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|
id="g661" />
|
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<g
|
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|
id="g663" />
|
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<g
|
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|
|
id="g665" />
|
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<g
|
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|
|
id="g667">
|
|
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|
|
|
<g
|
|
|
|
|
|
id="g669"
|
|
|
|
|
|
clip-path="url(#clipPath673)">
|
|
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|
|
|
<path
|
|
|
|
|
|
d="M 0,0 H 170.08 V 113.39 H 0 Z"
|
|
|
|
|
|
style="fill:#ffffff;fill-opacity:1;fill-rule:nonzero;stroke:#ffffff;stroke-width:1.07;stroke-linecap:round;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
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id="path675" />
|
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|
|
|
|
</g>
|
|
|
|
|
|
</g>
|
|
|
|
|
|
<g
|
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|
|
|
|
id="g677" />
|
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<g
|
|
|
|
|
|
id="g679">
|
|
|
|
|
|
<g
|
|
|
|
|
|
id="g681"
|
|
|
|
|
|
clip-path="url(#clipPath685)">
|
|
|
|
|
|
<path
|
|
|
|
|
|
d="m 31.56,23.47 h 70.41 v 84.44 H 31.56 Z"
|
|
|
|
|
|
style="fill:#ffffff;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
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|
|
id="path687" />
|
|
|
|
|
|
<path
|
|
|
|
|
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d="m 43.61,65.85 c 0,0.63 0.52,1.15 1.16,1.15 0.63,0 1.15,-0.52 1.15,-1.15 0,-0.64 -0.52,-1.16 -1.15,-1.16 -0.64,0 -1.16,0.52 -1.16,1.16"
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style="fill:#00bfc4;fill-opacity:1;fill-rule:nonzero;stroke:#00bfc4;stroke-width:0.71;stroke-linecap:round;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
|
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|
|
id="path689" />
|
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|
|
|
<path
|
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|
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d="m 56.61,80 c 0,0.63 0.52,1.15 1.16,1.15 0.63,0 1.15,-0.52 1.15,-1.15 0,-0.64 -0.52,-1.16 -1.15,-1.16 -0.64,0 -1.16,0.52 -1.16,1.16"
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style="fill:#00bfc4;fill-opacity:1;fill-rule:nonzero;stroke:#00bfc4;stroke-width:0.71;stroke-linecap:round;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
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id="path691" />
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<path
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sodipodi:role="line"
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id="tspan771">0%</tspan></text>
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sodipodi:role="line"
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<text
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sodipodi:role="line"
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sodipodi:role="line"
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id="tspan783">75%</tspan></text>
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<text
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transform="matrix(1,0,0,-1,13.85,102.27)"
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sodipodi:role="line"
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id="tspan787">100%</tspan></text>
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</g>
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<g
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id="path799" />
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<path
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<path
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id="g807" />
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id="g809" />
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id="g811" />
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<g
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id="g813" />
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<g
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id="g815" />
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<g
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id="g817">
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<path
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d="m 31.56,23.47 h 70.41"
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style="fill:none;stroke:#000000;stroke-width:0.43;stroke-linecap:butt;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
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</g>
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<g
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id="g821" />
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<g
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id="g823" />
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<g
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id="g825">
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<path
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d="m 34.76,20.73 v 2.74"
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style="fill:none;stroke:#333333;stroke-width:0.43;stroke-linecap:butt;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
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<path
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<path
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d="m 74.77,20.73 v 2.74"
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style="fill:none;stroke:#333333;stroke-width:0.43;stroke-linecap:butt;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
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<path
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d="m 94.77,20.73 v 2.74"
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style="fill:none;stroke:#333333;stroke-width:0.43;stroke-linecap:butt;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
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</g>
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<g
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<g
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id="g837">
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<text
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transform="matrix(1,0,0,-1,33.37,14.95)"
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style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#4d4d4d;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text841"><tspan
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x="0"
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y="0"
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id="tspan839">0</tspan></text>
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<text
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transform="matrix(1,0,0,-1,51.99,14.95)"
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style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#4d4d4d;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text845"><tspan
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x="0 2.78"
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y="0"
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sodipodi:role="line"
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id="tspan843">10</tspan></text>
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<text
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transform="matrix(1,0,0,-1,71.99,14.95)"
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style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#4d4d4d;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text849"><tspan
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x="0 2.78"
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y="0"
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sodipodi:role="line"
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id="tspan847">20</tspan></text>
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<text
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transform="matrix(1,0,0,-1,91.99,14.95)"
|
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|
style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#4d4d4d;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text853"><tspan
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x="0 2.78"
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y="0"
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sodipodi:role="line"
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id="tspan851">30</tspan></text>
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</g>
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<g
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id="g855" />
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<g
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id="g857" />
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<g
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id="g859" />
|
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<g
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id="g861" />
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<g
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|
id="g863" />
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<g
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id="g865">
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<text
|
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|
transform="matrix(1,0,0,-1,52.44,6.8)"
|
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|
|
style="font-variant:normal;font-weight:normal;font-size:6px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text869"><tspan
|
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x="0 3.6659999 4.9980001 9.9960003 13.332 15 16.997999 21.996 23.327999 26.664"
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y="0"
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sodipodi:role="line"
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id="tspan867">Time (min)</tspan></text>
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</g>
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<g
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id="g871" />
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<g
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id="g873">
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<text
|
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transform="matrix(0,1,1,0,9.79,43.16)"
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style="font-variant:normal;font-weight:normal;font-size:6px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text877"><tspan
|
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x="0 3.7019999 7.0380001 9.0360003 12.036 15.372 18.708 20.375999 22.044001 25.379999 27.048 28.716 32.051998 35.051998 38.388 41.723999"
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y="0"
|
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sodipodi:role="line"
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id="tspan875">Percent attached</tspan></text>
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</g>
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<g
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id="g879" />
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<g
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id="g881" />
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<g
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id="g883" />
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<g
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id="g885" />
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<g
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id="g887" />
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<g
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id="g889" />
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<g
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id="g891" />
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<g
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id="g893">
|
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<path
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d="M 107.64,47.38 H 164.6 V 83.99 H 107.64 Z"
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|
style="fill:#ffffff;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="path895" />
|
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</g>
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<g
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id="g897" />
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<g
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id="g899" />
|
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<g
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id="g901" />
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<g
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id="g903">
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<text
|
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|
transform="matrix(1,0,0,-1,110.48,76.19)"
|
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|
style="font-variant:normal;font-weight:normal;font-size:6px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text907"><tspan
|
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x="0 4.0019999 7.3379998 8.6700001 10.158 13.494 16.997999 21.33 25.662001 29.664 33.167999 37.169998 38.501999 41.838001 43.506001 44.838001"
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y="0"
|
|
|
|
|
|
sodipodi:role="line"
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|
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id="tspan905">Sulfo−NHS−Biotin</tspan></text>
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|
<text
|
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|
transform="matrix(1,0,0,-1,110.48,69.71)"
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style="font-variant:normal;font-weight:normal;font-size:6px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text911"><tspan
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x="0 4.3319998 7.6680002 11.004 14.004 17.34 20.676001 22.344 24.282 27.618 29.285999 30.618 33.953999 37.290001 38.958 40.956001 44.292 49.290001"
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y="0"
|
|
|
|
|
|
sodipodi:role="line"
|
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id="tspan909">Concentration (uM)</tspan></text>
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|
</g>
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<g
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id="g913" />
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<g
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id="g915" />
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<g
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id="g917" />
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<g
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id="g919" />
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<g
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id="g921">
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<path
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style="fill:#f8766d;fill-opacity:1;fill-rule:nonzero;stroke:#f8766d;stroke-width:0.71;stroke-linecap:round;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
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id="path923" />
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</g>
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<g
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<g
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id="g927">
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<path
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d="m 111.27,62.1 h 6.33"
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<g
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<g
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<g
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<g
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id="g937">
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<path
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style="fill:#00bfc4;fill-opacity:1;fill-rule:nonzero;stroke:#00bfc4;stroke-width:0.71;stroke-linecap:round;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
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</g>
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<g
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<g
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id="g943">
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<path
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</g>
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<g
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id="g947" />
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<g
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id="g949" />
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<g
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id="g951" />
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<g
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id="g953">
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<text
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transform="matrix(1,0,0,-1,121.39,60.3)"
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style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text957"><tspan
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x="0 2.78"
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y="0"
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sodipodi:role="line"
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id="tspan955">75</tspan></text>
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</g>
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<g
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id="g959" />
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<g
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id="g961" />
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<g
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id="g963" />
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<g
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id="g965" />
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<g
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id="g967">
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<text
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transform="matrix(1,0,0,-1,121.39,52.38)"
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style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text971"><tspan
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x="0 2.78 5.5599999"
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y="0"
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sodipodi:role="line"
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<path
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<path
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<path
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</g>
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<g
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<g
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id="g15406">
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<text
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transform="matrix(1,0,0,-1,26.79,14.95)"
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style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#4d4d4d;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text15410"><tspan
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x="0"
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y="0"
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id="tspan15408">0</tspan></text>
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<text
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transform="matrix(1,0,0,-1,44.38,14.95)"
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style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#4d4d4d;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text15414"><tspan
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x="0 2.78"
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y="0"
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sodipodi:role="line"
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id="tspan15412">25</tspan></text>
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<text
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transform="matrix(1,0,0,-1,63.35,14.95)"
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style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#4d4d4d;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text15418"><tspan
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x="0 2.78"
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y="0"
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sodipodi:role="line"
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id="tspan15416">50</tspan></text>
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<text
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transform="matrix(1,0,0,-1,82.32,14.95)"
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style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#4d4d4d;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text15422"><tspan
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x="0 2.78"
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y="0"
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sodipodi:role="line"
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id="tspan15420">75</tspan></text>
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<text
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transform="matrix(1,0,0,-1,99.91,14.95)"
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style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#4d4d4d;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text15426"><tspan
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x="0 2.78 5.5599999"
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y="0"
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sodipodi:role="line"
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id="tspan15424">100</tspan></text>
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</g>
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<g
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id="g15428" />
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<g
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id="g15430" />
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<g
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id="g15432" />
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<g
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id="g15434" />
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<g
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id="g15436" />
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<g
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id="g15438">
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<text
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transform="matrix(1,0,0,-1,40.11,6.8)"
|
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style="font-variant:normal;font-weight:normal;font-size:6px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text15442"><tspan
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x="0 4.0019999 5.3340001 8.6700001 10.338 11.67 15.006 16.674 20.01 23.346001 26.681999 30.018 33.354 35.021999 37.02 40.355999 45.354 48.689999 50.021999"
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y="0"
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sodipodi:role="line"
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id="tspan15440">Biotin added (nmol)</tspan></text>
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</g>
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<g
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id="g15444" />
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<g
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id="g15446">
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<text
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transform="matrix(0,1,1,0,9.79,34.51)"
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style="font-variant:normal;font-weight:normal;font-size:6px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
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id="text15450"><tspan
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x="0 4.0019999 5.3340001 8.6700001 10.338 11.67 15.006 16.674 18.006001 21.341999 23.01 26.01 29.346001 30.677999 34.014 35.681999 37.014 40.349998 43.686001 45.354 47.352001 50.688 55.686001 59.021999 60.354"
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y="0"
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|
sodipodi:role="line"
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id="tspan15448">Biotin in solution (nmol)</tspan></text>
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|
</g>
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<g
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id="g15452" />
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<g
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id="g15454" />
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<g
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id="g15456" />
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<g
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id="g15458" />
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<g
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id="g15460" />
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<g
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id="g15462" />
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<g
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id="g15464" />
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<g
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id="g15466" />
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<g
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id="g15468" />
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<g
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id="g15470" />
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<g
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id="g15472" />
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<g
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id="g15474" />
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</g>
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<text
|
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xml:space="preserve"
|
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|
|
style="font-size:16px;line-height:1.25;font-family:sans-serif;letter-spacing:0px"
|
|
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|
|
x="368.00003"
|
|
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|
|
y="178.00002"
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id="text16938"><tspan
|
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sodipodi:role="line"
|
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id="tspan16936"
|
|
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|
|
x="368.00003"
|
|
|
|
|
|
y="178.00002">d.</tspan></text>
|
2021-07-23 17:16:41 -04:00
|
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</g>
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</svg>
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