2021-08-03 19:01:31 -04:00
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\begin{verbatim}
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###Biotin washing###
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---Stage 1---
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initial bulk concentration = 879.6421 nM
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final bulk concentration = 986.9753 nM
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bulk percent change = 10.875%
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initial carrier concentration = 10000 nM
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initial carrier amount = 1.3197 nmol
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initial bulk amount = 8.6803 nmol
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final time to equilibrium = 3 min
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2021-08-03 19:54:21 -04:00
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Total removed from carriers = 1.1917 nmol
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Total remaining in carriers = 0.12791 nmol
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Total remaining in bulk = 9.8721 nmol
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Percent removed from carriers = 90.3071 %
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2021-08-03 19:01:31 -04:00
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---Stage 2---
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2021-08-03 19:54:21 -04:00
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initial bulk concentration = 56.8419 nM
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final bulk concentration = 67.986 nM
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2021-08-03 19:01:31 -04:00
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bulk percent change = 16.3917%
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2021-08-03 19:54:21 -04:00
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initial carrier concentration = 969.2905 nM
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initial carrier amount = 0.12791 nmol
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initial bulk amount = 0.56092 nmol
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2021-08-03 19:01:31 -04:00
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final time to equilibrium = 3 min
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2021-08-03 19:54:21 -04:00
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Total removed from carriers = 0.11919 nmol
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Total remaining in carriers = 0.0087244 nmol
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Total remaining in bulk = 0.68011 nmol
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Percent removed from carriers = 93.1795 %
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2021-08-03 19:01:31 -04:00
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---Stage 3---
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2021-08-03 19:54:21 -04:00
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initial bulk concentration = 3.8769 nM
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final bulk concentration = 4.637 nM
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2021-08-03 19:01:31 -04:00
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bulk percent change = 16.3917%
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2021-08-03 19:54:21 -04:00
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initial carrier concentration = 66.1106 nM
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initial carrier amount = 0.0087244 nmol
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initial bulk amount = 0.038257 nmol
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2021-08-03 19:01:31 -04:00
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final time to equilibrium = 3 min
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2021-08-03 19:54:21 -04:00
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Total removed from carriers = 0.0081293 nmol
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Total remaining in carriers = 0.00059505 nmol
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Total remaining in bulk = 0.046387 nmol
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Percent removed from carriers = 93.1795 %
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2021-08-03 19:01:31 -04:00
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2021-08-03 19:54:21 -04:00
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final bulk amount = 0.0030925 nmol
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final total amount = 0.0036875 nmol
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overall reduction = 99.9631 %
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2021-08-03 19:01:31 -04:00
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###Streptavidin washing###
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---Stage 1---
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initial bulk concentration = 23.9902 nM
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final bulk concentration = 26.9175 nM
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bulk percent change = 10.875%
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initial carrier concentration = 272.7273 nM
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initial carrier amount = 0.035991 nmol
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initial bulk amount = 0.23674 nmol
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2021-08-03 19:54:21 -04:00
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final time to equilibrium = 13 min
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Total removed from carriers = 0.032344 nmol
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Total remaining in carriers = 0.0036466 nmol
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Total remaining in bulk = 0.26908 nmol
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Percent removed from carriers = 89.8678 %
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2021-08-03 19:01:31 -04:00
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---Stage 2---
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2021-08-03 19:54:21 -04:00
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initial bulk concentration = 1.6205 nM
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final bulk concentration = 1.9382 nM
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2021-08-03 19:01:31 -04:00
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bulk percent change = 16.3917%
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2021-08-03 19:54:21 -04:00
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initial carrier concentration = 27.6332 nM
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initial carrier amount = 0.0036466 nmol
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initial bulk amount = 0.015991 nmol
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2021-08-03 19:01:31 -04:00
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2021-08-03 19:54:21 -04:00
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final time to equilibrium = 13 min
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Total removed from carriers = 0.0033814 nmol
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Total remaining in carriers = 0.00026528 nmol
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Total remaining in bulk = 0.019372 nmol
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Percent removed from carriers = 92.7253 %
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2021-08-03 19:01:31 -04:00
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2021-08-03 19:54:21 -04:00
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final bulk amount = 0.0012915 nmol
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final total amount = 0.0015568 nmol
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overall reduction = 99.4292 %
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2021-08-03 19:01:31 -04:00
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###Antibody washing###
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---Stage 1---
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initial bulk concentration = 0.58643 nM
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final bulk concentration = 0.65798 nM
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bulk percent change = 10.875%
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initial carrier concentration = 6.6667 nM
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initial carrier amount = 0.00087977 nmol
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initial bulk amount = 0.0057869 nmol
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2021-08-03 19:54:21 -04:00
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final time to equilibrium = 16 min
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Total removed from carriers = 0.00078979 nmol
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Total remaining in carriers = 8.9987e-05 nmol
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Total remaining in bulk = 0.0065767 nmol
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Percent removed from carriers = 89.7715 %
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2021-08-03 19:01:31 -04:00
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---Stage 2---
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2021-08-03 19:54:21 -04:00
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initial bulk concentration = 0.039988 nM
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final bulk concentration = 0.047828 nM
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2021-08-03 19:01:31 -04:00
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bulk percent change = 16.3917%
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2021-08-03 19:54:21 -04:00
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initial carrier concentration = 0.6819 nM
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initial carrier amount = 8.9987e-05 nmol
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initial bulk amount = 0.00039461 nmol
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final time to equilibrium = 17 min
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Total removed from carriers = 8.3517e-05 nmol
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Total remaining in carriers = 6.4705e-06 nmol
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Total remaining in bulk = 0.00047812 nmol
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Percent removed from carriers = 92.8096 %
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final bulk amount = 3.1875e-05 nmol
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final total amount = 3.8345e-05 nmol
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overall reduction = 99.4248 %
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2021-08-03 19:01:31 -04:00
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\end{verbatim}
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