diff --git a/tex/thesis.tex b/tex/thesis.tex index 4415035..9f4778b 100644 --- a/tex/thesis.tex +++ b/tex/thesis.tex @@ -572,23 +572,23 @@ approved T cell expansion technologies involve \acd{3} and \acd{28} \glspl{mab}, usually mounted on magnetic beads. This method fails to recapitulate many key signals found \invivo{} and is also heavily licensed by a few companies, limiting its long-term usefulness to manufactures and clinicians. Furthermore, -highly potent anti-tumor T cells are generally less-differentiated subtypes such -as \acrlongpl{tcm} and \acrlongpl{tscm}. Despite this understanding, little has -been done to optimize T cell expansion for generating these subtypes, including -measurement and feedback control strategies that are necessary for any modern -manufacturing process. +highly potent, anti-tumor T cells are generally less-differentiated subtypes +such as \acrlongpl{tcm} and \acrlongpl{tscm}. Despite this understanding, little +has been done to optimize T cell expansion for generating these subtypes, +including measurement and feedback control strategies that are necessary for any +modern manufacturing process. The goal of this dissertation was to develop a microcarrier-based \gls{dms} T cell expansion system and determine biologically-meaningful \glspl{cqa} and \glspl{cpp} that could be used to optimize for highly-potent T cells. In \cref{aim1}, we develop and characterized the \gls{dms} system, including -quality control steps. We also demonstrate the feasiblity of expanding +quality control steps. We also demonstrate the feasibility of expanding high-quality T cells. In \cref{aim2a,aim2b}, we use \gls{doe} methodology to -optimize the \gls{dms} platform, and develop a computational pipeline to -identify and model the effect of measurable \glspl{cqa}, and \glspl{cpp} on the +optimize the \gls{dms} platform, and we develop a computational pipeline to +identify and model the effects of measurable \glspl{cqa} and \glspl{cpp} on the final product. In \cref{aim3}, we demonstrate the effectiveness of the \gls{dms} platform \invivo{}. This thesis lays the groundwork for a novel T cell expansion -method which can be utilized at scale for a clinical trial and beyond. +method which can be utilized at scale for clinical trials and beyond. \clearpage