From 1719e545c2981be1e34269c915aa88646ce451de Mon Sep 17 00:00:00 2001 From: ndwarshuis Date: Fri, 30 Jul 2021 13:56:09 -0400 Subject: [PATCH] ENH add biotin wash fig ADD first doe fig --- figures/dms_timing.svg | 383 ++++++- figures/doe_responses_first.svg | 1765 +++++++++++++++++++++++++++++++ tex/thesis.tex | 21 +- 3 files changed, 2164 insertions(+), 5 deletions(-) create mode 100644 figures/doe_responses_first.svg diff --git a/figures/dms_timing.svg b/figures/dms_timing.svg index 41bf6d0..8567bde 100644 --- a/figures/dms_timing.svg +++ b/figures/dms_timing.svg @@ -46,6 +46,34 @@ d="m 31.56,23.47 h 70.41 v 84.44 H 31.56 Z" id="path683" /> + + + + + + + + + + + + c. + y="178.00002">c. + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 0 + 20 + 40 + 60 + 80 + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 0 + 25 + 50 + 75 + 100 + + + + + + + + Biotin added (nmol) + + + + Biotin in solution (nmol) + + + + + + + + + + + + + + + d. diff --git a/figures/doe_responses_first.svg b/figures/doe_responses_first.svg new file mode 100644 index 0000000..4964637 --- /dev/null +++ b/figures/doe_responses_first.svg @@ -0,0 +1,1765 @@ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + image/svg+xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 5.0e+061.0e+071.5e+072.0e+07 + + + + + + + + + + + 1015202530 + IL2 concentration(U/mL) + CCR7+CD62L+ cells + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 5001000150020002500 + DMS concentration(DMSs/mL) + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 60708090100 + Functional mAbDensity (%) + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 2e+064e+066e+06 + + + + + + + + + + 1015202530 + IL2 concentration(U/mL) + CD4+ cells + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 5001000150020002500 + DMS concentration(DMSs/mL) + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 60708090100 + Functional mAbDensity (%) + + + + + a. + b. + + diff --git a/tex/thesis.tex b/tex/thesis.tex index cfad655..39ed10e 100644 --- a/tex/thesis.tex +++ b/tex/thesis.tex @@ -1332,6 +1332,7 @@ the \gls{mab} reaction should proceed in {\#}{mab curve}. \phantomsubcaption\label{fig:dms_biotin_rxn_mass} \phantomsubcaption\label{fig:dms_biotin_rxn_frac} \phantomsubcaption\label{fig:dms_stp_per_time} + \phantomsubcaption\label{fig:dms_biotin_washed} \endgroup \caption[\gls{dms} Reaction kinetics] @@ -1339,6 +1340,8 @@ the \gls{mab} reaction should proceed in {\#}{mab curve}. \subcap{fig:dms_biotin_rxn_mass}{Biotin mass bound per time} \subcap{fig:dms_biotin_rxn_frac}{Fraction of input biotin bound per time} \subcap{fig:dms_stp_per_time}{\Gls{stp} bound per time.} + \subcap{fig:dms_biotin_washed}{Biotin quantification via the \gls{haba} + assay after washing.} } \label{fig:dms_kinetics} \end{figure*} @@ -2291,7 +2294,22 @@ at \SI{10}{\IU\per\ml} throughout the remainder of this aim. \input{../tables/doe_runs.tex} \end{table} -% FIGURE first DOE results to show how the second DOE was motivated +% RESULT integrate this figure into the results paragraph +\begin{figure*}[ht!] + \begingroup + + \includegraphics{../figures/doe_responses_first.png} + \phantomsubcaption\label{fig:doe_response_first_mem} + \phantomsubcaption\label{fig:doe_response_first_cd4} + + \endgroup + \caption[Response plots for first DOE] + {Response plots from the first \gls{doe} experiment for + \subcap{fig:doe_response_first_mem}{\ptmemp{}} and + \subcap{fig:doe_response_first_cd4}{\pthp{}}. Each point is one run. + } + \label{fig:doe_response_first} +\end{figure*} We conducted two consecutive \glspl{doe} to optimize the \pth{} and \ptmem{} responses for the \gls{dms} system. In the first \gls{doe} we, tested \pilII{} in @@ -2827,7 +2845,6 @@ are fundamentally altered by changing the number of \glspl{dms} temporally. \label{fig:add_rem} \end{figure*} - \begin{figure*}[ht!] \begingroup