diff --git a/.gitignore b/.gitignore index 0386601..febfc5a 100644 --- a/.gitignore +++ b/.gitignore @@ -1,9 +1,13 @@ * -!tex -tex/* -!tex/*.tex -!tex/*.bib -!tex/*.glo +!thesis +thesis/* +!thesis/*.tex +!thesis/*.bib +!thesis/*.glo + +!abstract +abstract/* +!abstract/*.tex !figures figures/* diff --git a/abstract/abstract.tex b/abstract/abstract.tex new file mode 100644 index 0000000..0eb002b --- /dev/null +++ b/abstract/abstract.tex @@ -0,0 +1,57 @@ +\documentclass{article} +\usepackage[top=1in,left=1in,right=1in,bottom=1in]{geometry} +\usepackage{setspace} + +\pagestyle{empty} + +\begin{document} + +\begin{center} + \LARGE{\textbf{Optimizing T Cell Manufacturing and Quality Using + Functionalized Degradable Microscaffolds}} + + \Large + + \bigskip + + Nathan J. Dwarshuis + + \bigskip + + 165 pages + + \bigskip + + Directed by Krishnendu Roy +\end{center} + +\large + +\doublespacing{} + +Adoptive cell therapy using chimeric antigen receptor (CAR) T cells have shown +promise in treating cancer, but manufacturing large numbers of high quality +cells remains challenging. Currently approved T cell expansion technologies +involve anti-CD3 and anti-CD28 antibodies, usually mounted on magnetic beads. +This method fails to recapitulate many key signals found \textit{in vivo} and is +also heavily licensed by a few companies, limiting its long-term usefulness to +manufactures and clinicians. Furthermore, highly potent, anti-tumor T cells are +generally less-differentiated subtypes such as central memory and stem memory T +cells. Despite this understanding, little has been done to optimize T cell +expansion for generating these subtypes, including measurement and feedback +control strategies that are necessary for any modern manufacturing process. + +The goal of this dissertation was to develop a microcarrier-based degradable +microscaffold (DMS) T cell expansion system and determine +biologically-meaningful critical quality attitudes and critical process +parameters that could be used to optimize for highly-potent T cells. We +developed and characterized the DMS system, including quality control steps. We +also demonstrated the feasibility of expanding high-quality T cells. We used +Design of Experiments methodology to optimize the DMS platform, and we developed +a computational pipeline to identify and model the effects of measurable +critical quality attributes and critical process parameters on the final +product. Finally, we demonstrated the effectiveness of the DMS platform +\textit{in vivo}. This thesis lays the groundwork for a novel T cell expansion +method which can be utilized at scale for clinical trials and beyond. + +\end{document} \ No newline at end of file diff --git a/tex/references.bib b/thesis/references.bib similarity index 100% rename from tex/references.bib rename to thesis/references.bib diff --git a/tex/thesis.glo b/thesis/thesis.glo similarity index 100% rename from tex/thesis.glo rename to thesis/thesis.glo diff --git a/tex/thesis.tex b/thesis/thesis.tex similarity index 100% rename from tex/thesis.tex rename to thesis/thesis.tex index ac39ef3..6971b15 100644 --- a/tex/thesis.tex +++ b/thesis/thesis.tex @@ -1,6 +1,6 @@ \documentclass{report} -\usepackage[section]{placeins} \usepackage[top=1in,left=1.5in,right=1in,bottom=1in]{geometry} +\usepackage[section]{placeins} \usepackage{siunitx} \usepackage{multicol} \setlength{\columnsep}{1cm}