diff --git a/tex/thesis.tex b/tex/thesis.tex index da9fc98..7139392 100644 --- a/tex/thesis.tex +++ b/tex/thesis.tex @@ -2393,8 +2393,10 @@ We performed the same experiments as described in \cref{fig:mouse_dosing_overview} with the modification that T cells were only grown and harvested after \SI{6}{\day}, \SI{10}{\day}, or \SI{14}{\day} of expansion (\cref{fig:mouse_timecourse_overview}). T cells were frozen after -harvest, and all timepoints were thawed at the same time prior to injection. All -other characteristics of the experiment were the same. +harvest, and all timepoints were thawed at the same time prior to injection. The +dose of T cells injected was \num{1.25e6} cells per mouse (the same as the high +dose in the first experiment). All other characteristics of the experiment were +the same. \begin{figure*}[ht!] \begingroup @@ -2486,6 +2488,9 @@ other groups in regard to the final tumor burden. \section{discussion} +% TABLE make a summary table showing the results from both experiments; this is +% tough to explain. + When we tested bead and DMS expanded \gls{car} T cells, we also found that the \gls{dms} expanded CAR-T cells outperformed bead groups in prolonging survival of Nalm-6 tumor challenged (intravenously injected) \gls{nsg} mice. DMS expanded @@ -2500,7 +2505,21 @@ results suggest that the higher proportion of memory T cells in DMS groups efficiently kill tumor cells as recently reported in literature\cite{Fraietta2018, Sommermeyer2015}. -% DISCUSSION 2nd mouse model +% TODO try and find literature explaining what the ideal ratio is +When testing \gls{car} T cells at earlier timepoints relative to day 14 as used +in the first \invivo{} experiment, we noted that none of the \gls{car} +treatments seemed to work as well as they did in the first experiment. However, +at day 14, we should note that the number of \gls{car} T cells injected in the +second experiment was lower than the lowest dose in the first for both bead and +\gls{dms} (\cref{fig:mouse_timecourse_qc_car,tab:mouse_dosing_results}). While +the \ptmemp{} generally increases with earlier timepoints in the second +experiment, the first experiment suggests that \ptmemp{} may not be the primary +driver in this particular model +(\cref{fig:mouse_timecourse_qc_mem,fig:mouse_dosing_qc_mem}). As with the first +experiment, the \pthp{} seems to be higher overall in the \gls{dms} group than +the bead group (\cref{fig:mouse_dosing_qc_cd4,fig:mouse_timecourse_qc_cd4}), and +this may explain the modest advantage that the \gls{dms} T cells seemed to have +in the second experiment in slowing the progression of tumor burden. \chapter{conclusions and future work}\label{conclusions} \section{conclusions}