ADD results for mouse 2 experiment
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<g
|
|
||||||
id="g102601" />
|
|
||||||
<g
|
|
||||||
id="g102603">
|
|
||||||
<text
|
|
||||||
transform="matrix(0,1,1,0,9.79,29.02)"
|
|
||||||
style="font-variant:normal;font-weight:normal;font-size:6px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
|
||||||
id="text102607"><tspan
|
|
||||||
x="0 4.3319998 8.3339996 12.666 16.17 20.502001 24.834 28.17 31.674 33.341999 38.675999 40.344002 42.341999 45.678001 47.346001 49.014 53.346001 57.678001 61.014 64.517998"
|
|
||||||
y="0"
|
|
||||||
sodipodi:role="line"
|
|
||||||
id="tspan102605">CAR+CD4+ % (of CD3+)</tspan></text>
|
|
||||||
</g>
|
|
||||||
<g
|
|
||||||
id="g102609" />
|
|
||||||
<g
|
|
||||||
id="g102611" />
|
|
||||||
<g
|
|
||||||
id="g102613" />
|
|
||||||
<g
|
|
||||||
id="g102615" />
|
|
||||||
<g
|
|
||||||
id="g102617" />
|
|
||||||
<g
|
|
||||||
id="g102619" />
|
|
||||||
<g
|
|
||||||
id="g102621" />
|
|
||||||
<g
|
|
||||||
id="g102623">
|
|
||||||
<path
|
|
||||||
d="m 122.17,46.55 h 28.25 V 78 h -28.25 z"
|
|
||||||
style="fill:#ffffff;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
|
||||||
id="path102625" />
|
|
||||||
</g>
|
|
||||||
<g
|
|
||||||
id="g102627" />
|
|
||||||
<g
|
|
||||||
id="g102629" />
|
|
||||||
<g
|
|
||||||
id="g102631" />
|
|
||||||
<g
|
|
||||||
id="g102633">
|
|
||||||
<text
|
|
||||||
transform="matrix(1,0,0,-1,125.01,69.53)"
|
|
||||||
style="font-variant:normal;font-weight:normal;font-size:6px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
|
||||||
id="text102637"><tspan
|
|
||||||
x="0 4.9980001 8.3339996 10.002 13.338 16.674"
|
|
||||||
y="0"
|
|
||||||
sodipodi:role="line"
|
|
||||||
id="tspan102635">Method</tspan></text>
|
|
||||||
</g>
|
|
||||||
<g
|
|
||||||
id="g102639" />
|
|
||||||
<g
|
|
||||||
id="g102641" />
|
|
||||||
<g
|
|
||||||
id="g102643" />
|
|
||||||
<g
|
|
||||||
id="g102645" />
|
|
||||||
<g
|
|
||||||
id="g102647">
|
|
||||||
<path
|
|
||||||
d="m 125.72,58.01 h 6.5 v 6.5 h -6.5 z"
|
|
||||||
style="fill:#f8766d;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
|
||||||
id="path102649" />
|
|
||||||
</g>
|
|
||||||
<g
|
|
||||||
id="g102651" />
|
|
||||||
<g
|
|
||||||
id="g102653" />
|
|
||||||
<g
|
|
||||||
id="g102655" />
|
|
||||||
<g
|
|
||||||
id="g102657">
|
|
||||||
<path
|
|
||||||
d="m 125.72,50.09 h 6.5 v 6.5 h -6.5 z"
|
|
||||||
style="fill:#00bfc4;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
|
||||||
id="path102659" />
|
|
||||||
</g>
|
|
||||||
<g
|
|
||||||
id="g102661" />
|
|
||||||
<g
|
|
||||||
id="g102663" />
|
|
||||||
<g
|
|
||||||
id="g102665" />
|
|
||||||
<g
|
|
||||||
id="g102667">
|
|
||||||
<text
|
|
||||||
transform="matrix(1,0,0,-1,135.92,59.47)"
|
|
||||||
style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
|
||||||
id="text102671"><tspan
|
|
||||||
x="0 3.335 6.1149998 8.8950005"
|
|
||||||
y="0"
|
|
||||||
sodipodi:role="line"
|
|
||||||
id="tspan102669">Bead</tspan></text>
|
|
||||||
</g>
|
|
||||||
<g
|
|
||||||
id="g102673" />
|
|
||||||
<g
|
|
||||||
id="g102675" />
|
|
||||||
<g
|
|
||||||
id="g102677" />
|
|
||||||
<g
|
|
||||||
id="g102679" />
|
|
||||||
<g
|
|
||||||
id="g102681">
|
|
||||||
<text
|
|
||||||
transform="matrix(1,0,0,-1,135.92,51.55)"
|
|
||||||
style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
|
||||||
id="text102685"><tspan
|
|
||||||
x="0 3.6099999 7.7750001"
|
|
||||||
y="0"
|
|
||||||
sodipodi:role="line"
|
|
||||||
id="tspan102683">DMS</tspan></text>
|
|
||||||
</g>
|
|
||||||
<g
|
|
||||||
id="g102687" />
|
|
||||||
<g
|
|
||||||
id="g102689" />
|
|
||||||
<g
|
|
||||||
id="g102691" />
|
|
||||||
<g
|
|
||||||
id="g102693" />
|
|
||||||
<g
|
|
||||||
id="g102695" />
|
|
||||||
<g
|
|
||||||
id="g102697" />
|
|
||||||
<g
|
|
||||||
id="g102699" />
|
|
||||||
<g
|
|
||||||
id="g102701" />
|
|
||||||
<g
|
|
||||||
id="g102703" />
|
|
||||||
<g
|
|
||||||
id="g102705" />
|
|
||||||
<g
|
|
||||||
id="g102707" />
|
|
||||||
<g
|
|
||||||
id="g102709" />
|
|
||||||
<g
|
|
||||||
id="g102711" />
|
|
||||||
</g>
|
|
||||||
<g
|
<g
|
||||||
id="g102885"
|
id="g102885"
|
||||||
inkscape:label="growth"
|
inkscape:label="growth"
|
||||||
|
|
@ -2080,5 +1690,397 @@
|
||||||
id="tspan110713"
|
id="tspan110713"
|
||||||
x="226.00002"
|
x="226.00002"
|
||||||
y="180.00002">d.</tspan></text>
|
y="180.00002">d.</tspan></text>
|
||||||
|
<g
|
||||||
|
id="g1654"
|
||||||
|
inkscape:label="cd4_fraction"
|
||||||
|
transform="matrix(1.3333333,0,0,-1.3333333,12.306673,336.53335)">
|
||||||
|
<g
|
||||||
|
id="g1656" />
|
||||||
|
<g
|
||||||
|
id="g1658" />
|
||||||
|
<g
|
||||||
|
id="g1660" />
|
||||||
|
<g
|
||||||
|
id="g1662" />
|
||||||
|
<g
|
||||||
|
id="g1664" />
|
||||||
|
<g
|
||||||
|
id="g1666" />
|
||||||
|
<g
|
||||||
|
id="g1668" />
|
||||||
|
<g
|
||||||
|
id="g1670" />
|
||||||
|
<g
|
||||||
|
id="g1672" />
|
||||||
|
<g
|
||||||
|
id="g1674" />
|
||||||
|
<g
|
||||||
|
id="g1676" />
|
||||||
|
<g
|
||||||
|
id="g1678" />
|
||||||
|
<g
|
||||||
|
id="g1680" />
|
||||||
|
<g
|
||||||
|
id="g1682" />
|
||||||
|
<g
|
||||||
|
id="g1684">
|
||||||
|
<g
|
||||||
|
id="g1686"
|
||||||
|
clip-path="url(#clipPath1690)">
|
||||||
|
<path
|
||||||
|
d="M 0,0 H 155.91 V 113.39 H 0 Z"
|
||||||
|
style="fill:#ffffff;fill-opacity:1;fill-rule:nonzero;stroke:#ffffff;stroke-width:1.07;stroke-linecap:round;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
|
||||||
|
id="path1692" />
|
||||||
|
</g>
|
||||||
|
</g>
|
||||||
|
<g
|
||||||
|
id="g1694" />
|
||||||
|
<g
|
||||||
|
id="g1696">
|
||||||
|
<g
|
||||||
|
id="g1698"
|
||||||
|
clip-path="url(#clipPath1702)">
|
||||||
|
<path
|
||||||
|
d="m 34.27,16.64 h 82.23 v 91.27 H 34.27 Z"
|
||||||
|
style="fill:#ffffff;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="path1704" />
|
||||||
|
<path
|
||||||
|
d="m 49.69,20.79 h 11.56 v 82.97 H 49.69 Z"
|
||||||
|
style="fill:#00bfc4;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="path1706" />
|
||||||
|
<path
|
||||||
|
d="m 38.13,20.79 h 11.56 v 65.3 H 38.13 Z"
|
||||||
|
style="fill:#f8766d;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="path1708" />
|
||||||
|
<path
|
||||||
|
d="M 75.39,20.79 H 86.95 V 88.45 H 75.39 Z"
|
||||||
|
style="fill:#00bfc4;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="path1710" />
|
||||||
|
<path
|
||||||
|
d="M 63.83,20.79 H 75.39 V 68.1 H 63.83 Z"
|
||||||
|
style="fill:#f8766d;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="path1712" />
|
||||||
|
<path
|
||||||
|
d="m 101.09,20.79 h 11.56 v 48.05 h -11.56 z"
|
||||||
|
style="fill:#00bfc4;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="path1714" />
|
||||||
|
<path
|
||||||
|
d="m 89.52,20.79 h 11.56 V 64 H 89.52 Z"
|
||||||
|
style="fill:#f8766d;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="path1716" />
|
||||||
|
</g>
|
||||||
|
</g>
|
||||||
|
<g
|
||||||
|
id="g1718" />
|
||||||
|
<g
|
||||||
|
id="g1720" />
|
||||||
|
<g
|
||||||
|
id="g1722" />
|
||||||
|
<g
|
||||||
|
id="g1724" />
|
||||||
|
<g
|
||||||
|
id="g1726" />
|
||||||
|
<g
|
||||||
|
id="g1728" />
|
||||||
|
<g
|
||||||
|
id="g1730" />
|
||||||
|
<g
|
||||||
|
id="g1732" />
|
||||||
|
<g
|
||||||
|
id="g1734" />
|
||||||
|
<g
|
||||||
|
id="g1736" />
|
||||||
|
<g
|
||||||
|
id="g1738" />
|
||||||
|
<g
|
||||||
|
id="g1740" />
|
||||||
|
<g
|
||||||
|
id="g1742">
|
||||||
|
<path
|
||||||
|
d="m 34.27,16.64 v 91.27"
|
||||||
|
style="fill:none;stroke:#000000;stroke-width:0.43;stroke-linecap:butt;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
|
||||||
|
id="path1744" />
|
||||||
|
</g>
|
||||||
|
<g
|
||||||
|
id="g1746" />
|
||||||
|
<g
|
||||||
|
id="g1748" />
|
||||||
|
<g
|
||||||
|
id="g1750">
|
||||||
|
<text
|
||||||
|
transform="matrix(1,0,0,-1,17.95,18.99)"
|
||||||
|
style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#4d4d4d;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="text1754"><tspan
|
||||||
|
x="0 2.78 4.1700001 6.9499998"
|
||||||
|
y="0"
|
||||||
|
sodipodi:role="line"
|
||||||
|
id="tspan1752">0.0%</tspan></text>
|
||||||
|
<text
|
||||||
|
transform="matrix(1,0,0,-1,15.17,47.82)"
|
||||||
|
style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#4d4d4d;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="text1758"><tspan
|
||||||
|
x="0 2.78 5.5599999 6.9499998 9.7299995"
|
||||||
|
y="0"
|
||||||
|
sodipodi:role="line"
|
||||||
|
id="tspan1756">20.0%</tspan></text>
|
||||||
|
<text
|
||||||
|
transform="matrix(1,0,0,-1,15.17,76.65)"
|
||||||
|
style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#4d4d4d;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="text1762"><tspan
|
||||||
|
x="0 2.78 5.5599999 6.9499998 9.7299995"
|
||||||
|
y="0"
|
||||||
|
sodipodi:role="line"
|
||||||
|
id="tspan1760">40.0%</tspan></text>
|
||||||
|
<text
|
||||||
|
transform="matrix(1,0,0,-1,15.17,105.48)"
|
||||||
|
style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#4d4d4d;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="text1766"><tspan
|
||||||
|
x="0 2.78 5.5599999 6.9499998 9.7299995"
|
||||||
|
y="0"
|
||||||
|
sodipodi:role="line"
|
||||||
|
id="tspan1764">60.0%</tspan></text>
|
||||||
|
</g>
|
||||||
|
<g
|
||||||
|
id="g1768" />
|
||||||
|
<g
|
||||||
|
id="g1770">
|
||||||
|
<path
|
||||||
|
d="m 31.53,20.79 h 2.74"
|
||||||
|
style="fill:none;stroke:#333333;stroke-width:0.43;stroke-linecap:butt;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
|
||||||
|
id="path1772" />
|
||||||
|
<path
|
||||||
|
d="m 31.53,49.62 h 2.74"
|
||||||
|
style="fill:none;stroke:#333333;stroke-width:0.43;stroke-linecap:butt;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
|
||||||
|
id="path1774" />
|
||||||
|
<path
|
||||||
|
d="m 31.53,78.44 h 2.74"
|
||||||
|
style="fill:none;stroke:#333333;stroke-width:0.43;stroke-linecap:butt;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
|
||||||
|
id="path1776" />
|
||||||
|
<path
|
||||||
|
d="m 31.53,107.27 h 2.74"
|
||||||
|
style="fill:none;stroke:#333333;stroke-width:0.43;stroke-linecap:butt;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
|
||||||
|
id="path1778" />
|
||||||
|
</g>
|
||||||
|
<g
|
||||||
|
id="g1780" />
|
||||||
|
<g
|
||||||
|
id="g1782" />
|
||||||
|
<g
|
||||||
|
id="g1784" />
|
||||||
|
<g
|
||||||
|
id="g1786" />
|
||||||
|
<g
|
||||||
|
id="g1788" />
|
||||||
|
<g
|
||||||
|
id="g1790" />
|
||||||
|
<g
|
||||||
|
id="g1792">
|
||||||
|
<path
|
||||||
|
d="M 34.27,16.64 H 116.5"
|
||||||
|
style="fill:none;stroke:#000000;stroke-width:0.43;stroke-linecap:butt;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
|
||||||
|
id="path1794" />
|
||||||
|
</g>
|
||||||
|
<g
|
||||||
|
id="g1796" />
|
||||||
|
<g
|
||||||
|
id="g1798" />
|
||||||
|
<g
|
||||||
|
id="g1800">
|
||||||
|
<path
|
||||||
|
d="m 49.69,13.9 v 2.74"
|
||||||
|
style="fill:none;stroke:#333333;stroke-width:0.43;stroke-linecap:butt;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
|
||||||
|
id="path1802" />
|
||||||
|
<path
|
||||||
|
d="m 75.39,13.9 v 2.74"
|
||||||
|
style="fill:none;stroke:#333333;stroke-width:0.43;stroke-linecap:butt;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
|
||||||
|
id="path1804" />
|
||||||
|
<path
|
||||||
|
d="m 101.09,13.9 v 2.74"
|
||||||
|
style="fill:none;stroke:#333333;stroke-width:0.43;stroke-linecap:butt;stroke-linejoin:round;stroke-miterlimit:10;stroke-dasharray:none;stroke-opacity:1"
|
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|
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|
||||||
|
</g>
|
||||||
|
<g
|
||||||
|
id="g1808" />
|
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<g
|
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id="g1810">
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<text
|
||||||
|
transform="matrix(1,0,0,-1,43.24,8.12)"
|
||||||
|
style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#4d4d4d;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="text1814"><tspan
|
||||||
|
x="0 3.6099999 6.2399998 8.7399998 10.13"
|
||||||
|
y="0"
|
||||||
|
sodipodi:role="line"
|
||||||
|
id="tspan1812">Day 6</tspan></text>
|
||||||
|
<text
|
||||||
|
transform="matrix(1,0,0,-1,67.54,8.12)"
|
||||||
|
style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#4d4d4d;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="text1818"><tspan
|
||||||
|
x="0 3.6099999 6.2399998 8.7399998 10.13 12.91"
|
||||||
|
y="0"
|
||||||
|
sodipodi:role="line"
|
||||||
|
id="tspan1816">Day 10</tspan></text>
|
||||||
|
<text
|
||||||
|
transform="matrix(1,0,0,-1,93.24,8.12)"
|
||||||
|
style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#4d4d4d;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
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|
id="text1822"><tspan
|
||||||
|
x="0 3.6099999 6.2399998 8.7399998 10.13 12.91"
|
||||||
|
y="0"
|
||||||
|
sodipodi:role="line"
|
||||||
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id="tspan1820">Day 14</tspan></text>
|
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|
</g>
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id="g1824" />
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id="g1826" />
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id="g1828" />
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id="g1830" />
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id="g1832" />
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id="g1834" />
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<g
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id="g1836" />
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<g
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id="g1838">
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<text
|
||||||
|
transform="matrix(0,1,1,0,9.79,30.71)"
|
||||||
|
style="font-variant:normal;font-weight:normal;font-size:6px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="text1842"><tspan
|
||||||
|
x="0 4.3319998 8.6639996 12 13.668 17.334 19.271999 22.608 25.608 27.275999 28.608 31.944 35.279999 36.948002 38.945999 42.282001 43.950001 45.618 49.950001 54.282001 57.618 61.122002"
|
||||||
|
y="0"
|
||||||
|
sodipodi:role="line"
|
||||||
|
id="tspan1840">CD4 Fraction (of CD3+)</tspan></text>
|
||||||
|
</g>
|
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|
<g
|
||||||
|
id="g1844" />
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id="g1846" />
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|
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id="g1848" />
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<g
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id="g1850" />
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id="g1852" />
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id="g1854" />
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id="g1856" />
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<g
|
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|
id="g1858">
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||||||
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<path
|
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|
d="m 122.17,46.55 h 28.25 V 78 h -28.25 z"
|
||||||
|
style="fill:#ffffff;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="path1860" />
|
||||||
|
</g>
|
||||||
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<g
|
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id="g1862" />
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<g
|
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id="g1864" />
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<g
|
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|
id="g1866" />
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<g
|
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|
id="g1868">
|
||||||
|
<text
|
||||||
|
transform="matrix(1,0,0,-1,125.01,69.53)"
|
||||||
|
style="font-variant:normal;font-weight:normal;font-size:6px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
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|
id="text1872"><tspan
|
||||||
|
x="0 4.9980001 8.3339996 10.002 13.338 16.674"
|
||||||
|
y="0"
|
||||||
|
sodipodi:role="line"
|
||||||
|
id="tspan1870">Method</tspan></text>
|
||||||
|
</g>
|
||||||
|
<g
|
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|
id="g1874" />
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<g
|
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|
id="g1876" />
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<g
|
||||||
|
id="g1878" />
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<g
|
||||||
|
id="g1880" />
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||||||
|
<g
|
||||||
|
id="g1882">
|
||||||
|
<path
|
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|
d="m 125.72,58.01 h 6.5 v 6.5 h -6.5 z"
|
||||||
|
style="fill:#f8766d;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="path1884" />
|
||||||
|
</g>
|
||||||
|
<g
|
||||||
|
id="g1886" />
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|
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id="g1888" />
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|
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|
id="g1890" />
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|
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id="g1892">
|
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<path
|
||||||
|
d="m 125.72,50.09 h 6.5 v 6.5 h -6.5 z"
|
||||||
|
style="fill:#00bfc4;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="path1894" />
|
||||||
|
</g>
|
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|
<g
|
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id="g1896" />
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|
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id="g1898" />
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|
<g
|
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|
id="g1900" />
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|
<g
|
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|
id="g1902">
|
||||||
|
<text
|
||||||
|
transform="matrix(1,0,0,-1,135.92,59.47)"
|
||||||
|
style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="text1906"><tspan
|
||||||
|
x="0 3.335 6.1149998 8.8950005"
|
||||||
|
y="0"
|
||||||
|
sodipodi:role="line"
|
||||||
|
id="tspan1904">Bead</tspan></text>
|
||||||
|
</g>
|
||||||
|
<g
|
||||||
|
id="g1908" />
|
||||||
|
<g
|
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id="g1910" />
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|
<g
|
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|
id="g1912" />
|
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<g
|
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|
id="g1914" />
|
||||||
|
<g
|
||||||
|
id="g1916">
|
||||||
|
<text
|
||||||
|
transform="matrix(1,0,0,-1,135.92,51.55)"
|
||||||
|
style="font-variant:normal;font-weight:normal;font-size:5px;font-family:Helvetica;-inkscape-font-specification:Helvetica;writing-mode:lr-tb;fill:#000000;fill-opacity:1;fill-rule:nonzero;stroke:none"
|
||||||
|
id="text1920"><tspan
|
||||||
|
x="0 3.6099999 7.7750001"
|
||||||
|
y="0"
|
||||||
|
sodipodi:role="line"
|
||||||
|
id="tspan1918">DMS</tspan></text>
|
||||||
|
</g>
|
||||||
|
<g
|
||||||
|
id="g1922" />
|
||||||
|
<g
|
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|
id="g1924" />
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|
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|
id="g1926" />
|
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|
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|
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|
id="g1928" />
|
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|
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|
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id="g1930" />
|
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<g
|
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|
id="g1932" />
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|
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|
id="g1934" />
|
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|
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|
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|
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|
id="g1938" />
|
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|
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|
id="g1940" />
|
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|
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|
id="g1942" />
|
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|
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|
||||||
|
id="g1944" />
|
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|
<g
|
||||||
|
id="g1946" />
|
||||||
|
</g>
|
||||||
</g>
|
</g>
|
||||||
</svg>
|
</svg>
|
||||||
|
|
|
||||||
|
Before Width: | Height: | Size: 79 KiB After Width: | Height: | Size: 78 KiB |
|
|
@ -102,6 +102,9 @@
|
||||||
\newacronym{tnfa}{TNF$\upalpha$}{tumor necrosis factor-$\upalpha$}
|
\newacronym{tnfa}{TNF$\upalpha$}{tumor necrosis factor-$\upalpha$}
|
||||||
\newacronym{sql}{SQL}{structured query language}
|
\newacronym{sql}{SQL}{structured query language}
|
||||||
\newacronym{fcs}{FCS}{flow cytometry standard}
|
\newacronym{fcs}{FCS}{flow cytometry standard}
|
||||||
|
\newacronym{ivis}{ivis}{in vivo imaging system}
|
||||||
|
\newacronym{iacuc}{IACUC}{institutional animal care and use committee}
|
||||||
|
|
||||||
|
|
||||||
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
|
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
|
||||||
% SI units for uber nerds
|
% SI units for uber nerds
|
||||||
|
|
@ -2232,18 +2235,18 @@ day 0 (-7 day relative to T cell injection), 1e6 firefly luciferase-expressing
|
||||||
via tail vein into each mouse. At day 7, saline or CAR T cells at the indicated
|
via tail vein into each mouse. At day 7, saline or CAR T cells at the indicated
|
||||||
doses from either bead or DMS-expanded T cell cultures (for 14 days) were
|
doses from either bead or DMS-expanded T cell cultures (for 14 days) were
|
||||||
injected into each mouse via tail vein. Tumor burden was quantified
|
injected into each mouse via tail vein. Tumor burden was quantified
|
||||||
longitudinally via IVIS Spectrum In Vivo Imaging System (Perkin Elmer). Briefly,
|
longitudinally via an \gls{ivis} Spectrum (Perkin Elmer). Briefly, 200ug/mice
|
||||||
200ug/mice luciferin at 15 mg/ml in PBS was injected intraperitoneally under
|
luciferin at 15 mg/ml in PBS was injected intraperitoneally under isoflurane
|
||||||
isoflurane anesthesia into each mouse and waited for at least 10 minutes before
|
anesthesia into each mouse and waited for at least 10 minutes before imaging.
|
||||||
imaging. Mice were anesthetized again and imaged using the IVIS. Mice from each
|
Mice were anesthetized again and imaged using the \gls{ivis}. Mice from each
|
||||||
treatment group/dose were anesthetized, injected, and imaged together, and
|
treatment group/dose were anesthetized, injected, and imaged together, and
|
||||||
exposure time of the IVIS was limited to avoid saturation based on the signal
|
exposure time of the \gls{ivis} was limited to avoid saturation based on the
|
||||||
from the saline group. IVIS images were processed by normalizing them to common
|
signal from the saline group. \gls{ivis} images were processed by normalizing
|
||||||
minimum and maximum photon counts and total flux was estimated in terms of
|
them to common minimum and maximum photon counts and total flux was estimated in
|
||||||
photons/second. Endpoint for each mouse was determined by IACUC euthanasia
|
terms of photons/second. Endpoint for each mouse was determined by \gls{iacuc}
|
||||||
criteria (hunched back, paralysis, blindness, lethargy, and weight loss).
|
euthanasia criteria (hunched back, paralysis, blindness, lethargy, and weight
|
||||||
Mice were euthanized according to these endpoint criteria using carbon dioxide
|
loss). Mice were euthanized according to these endpoint criteria using carbon
|
||||||
asphyxiation.
|
dioxide asphyxiation.
|
||||||
|
|
||||||
\subsection{statistics}
|
\subsection{statistics}
|
||||||
|
|
||||||
|
|
@ -2384,6 +2387,15 @@ case of beads (\cref{fig:mouse_dosing_qc_mem}).
|
||||||
|
|
||||||
\subsection{Beads and DMSs perform similarly at earlier timepoints}
|
\subsection{Beads and DMSs perform similarly at earlier timepoints}
|
||||||
|
|
||||||
|
We then asked how T cells harvested using either beads or \gls{dms} performed
|
||||||
|
when harvested at earlier timepoints {\#}{levine paper with early timepoints}.
|
||||||
|
We performed the same experiments as described in
|
||||||
|
\cref{fig:mouse_dosing_overview} with the modification that T cells were only
|
||||||
|
grown and harvested after \SI{6}{\day}, \SI{10}{\day}, or \SI{14}{\day} of
|
||||||
|
expansion (\cref{fig:mouse_timecourse_overview}). T cells were frozen after
|
||||||
|
harvest, and all timepoints were thawed at the same time prior to injection. All
|
||||||
|
other characteristics of the experiment were the same.
|
||||||
|
|
||||||
\begin{figure*}[ht!]
|
\begin{figure*}[ht!]
|
||||||
\begingroup
|
\begingroup
|
||||||
|
|
||||||
|
|
@ -2397,6 +2409,22 @@ case of beads (\cref{fig:mouse_dosing_qc_mem}).
|
||||||
\label{fig:mouse_timecourse_overview}
|
\label{fig:mouse_timecourse_overview}
|
||||||
\end{figure*}
|
\end{figure*}
|
||||||
|
|
||||||
|
% TODO find literature saying that CAR T cells grow slower
|
||||||
|
As was the case with the first \invivo{} experiment, T cells activated with
|
||||||
|
\glspl{dms} expanded much more efficiently compared to those expanded with beads
|
||||||
|
(\cref{fig:mouse_timecourse_qc_growth}). When we quantified the \ptcarp{} of T
|
||||||
|
cells harvested at each timepoint, we noted that the bead group had much higher
|
||||||
|
\ptcar{} expression at earlier timpoints compared to \gls{dms}, while they
|
||||||
|
equalized at later timepoints (\cref{fig:mouse_timecourse_qc_car}). In addition,
|
||||||
|
overall \ptcar{} expression decreased at later timepoints, indicating that
|
||||||
|
\gls{car} transduced T cells either grow slower or die faster compared to
|
||||||
|
untransduced cells. The \pthp{} of the harvested T cells was higher overall in
|
||||||
|
\gls{dms} expanded T cells but decreased with increasing timepoints
|
||||||
|
(\cref{fig:mouse_timecourse_qc_cd4}). The \ptmemp{} was similar at day 6
|
||||||
|
between bead and \gls{dms} groups but the \gls{dms} group had higher \ptmemp{}
|
||||||
|
at day 14 despite the overall \ptmemp{} decreasing with time as shown elsewhere
|
||||||
|
(\cref{fig:mouse_timecourse_qc_mem}) {\#}{levine paper mem over time}.
|
||||||
|
|
||||||
\begin{figure*}[ht!]
|
\begin{figure*}[ht!]
|
||||||
\begingroup
|
\begingroup
|
||||||
|
|
||||||
|
|
@ -2420,6 +2448,21 @@ case of beads (\cref{fig:mouse_dosing_qc_mem}).
|
||||||
\label{fig:mouse_timecourse_qc}
|
\label{fig:mouse_timecourse_qc}
|
||||||
\end{figure*}
|
\end{figure*}
|
||||||
|
|
||||||
|
We analyzed the tumor burden using \gls{ivis} which showed that mice that
|
||||||
|
received T cells from any group performed better than those that received only
|
||||||
|
saline (\cref{fig:mouse_timecourse_ivis}). Note that unlike the previous
|
||||||
|
experiment, many of the mice survived until day 40 at which point \gls{gvhd}
|
||||||
|
began to take effect (after euthanizing the mice at day 42, most had small or no
|
||||||
|
spleen). When comparing bead and \gls{dms} groups, the \gls{dms} T cells still
|
||||||
|
seemed superior to the bead group, at least initially (note that in this case
|
||||||
|
they had similar numbers of \ptcar{} cells). At day 6, both \gls{dms} and bead
|
||||||
|
groups seemed to eradicate the tumor initially, after which it came back after
|
||||||
|
day 21 for the bead and day 28 for the \gls{dms} group. The day 10 groups
|
||||||
|
performed somewhere in between, where they increased linearly unlike the day 6
|
||||||
|
groups but not as quickly as the day 14 groups. In the case of the \gls{dms} day
|
||||||
|
10 group, it also appeared like a few mice actually performed better than all
|
||||||
|
other groups in regard to the final tumor burden.
|
||||||
|
|
||||||
\begin{figure*}[ht!]
|
\begin{figure*}[ht!]
|
||||||
\begingroup
|
\begingroup
|
||||||
|
|
||||||
|
|
|
||||||
Loading…
Reference in New Issue