From 994df04db2210f5200489ddb5b3793f1ecb8c722 Mon Sep 17 00:00:00 2001 From: ndwarshuis Date: Fri, 30 Jul 2021 17:04:13 -0400 Subject: [PATCH] ADD experimental overview to integrin figs --- figures/integrin_1.svg | 190 ++++++++++++++++++++++++++++--- figures/integrin_2.svg | 252 ++++++++++++++++++++++++++++++++++++++--- tex/thesis.tex | 14 ++- 3 files changed, 427 insertions(+), 29 deletions(-) diff --git a/figures/integrin_1.svg b/figures/integrin_1.svg index 9363d49..17ad340 100644 --- a/figures/integrin_1.svg +++ b/figures/integrin_1.svg @@ -18,6 +18,48 @@ xmlns:dc="http://purl.org/dc/elements/1.1/"> + + + + + + + + + @@ -68,9 +110,9 @@ borderopacity="1.0" inkscape:pageopacity="1" inkscape:pageshadow="2" - inkscape:zoom="1.979899" - inkscape:cx="258.85159" - inkscape:cy="177.02923" + inkscape:zoom="3.959798" + inkscape:cx="139.90613" + inkscape:cy="86.999387" inkscape:document-units="px" inkscape:current-layer="layer1" showgrid="true" @@ -108,7 +150,7 @@ + transform="matrix(1.3333333,0,0,-1.3333333,240.0267,160.6)"> + transform="matrix(1.3333333,0,0,-1.3333333,9.9200128,321.65335)"> + transform="matrix(1.3333333,0,0,-1.3333333,236.30669,328.73335)"> b. + x="1.6358956" + y="170.45758">c. c. + x="224.57033" + y="170.45758">d. + + + + + + + + + + + + D0 + D6 + D14 + Activate + Harvest + + Add anti-CD49a/b(10mg/ml) + b. diff --git a/figures/integrin_2.svg b/figures/integrin_2.svg index 7a8a6aa..cc99340 100644 --- a/figures/integrin_2.svg +++ b/figures/integrin_2.svg @@ -46,6 +46,76 @@ d="m 43.53,21.82 h 90.2 v 86.08 h -90.2 z" id="path27191" /> + + + + + + + + + + + + + + + + transform="matrix(1.3333333,0,0,-1.3333333,6.600013,271.86668)"> + transform="matrix(1.3333333,0,0,-1.3333333,245.81337,273.50668)"> a. + x="-1.453125" + y="121.64001">b. a. + b. + x="239.0667" + y="121.64001">c. + + + + + + + + + + + + D0 + D6 + D4 + D2 + D14 + Activate + Harvest + + + + + Add anti-CD49a/b(10mg/ml) diff --git a/tex/thesis.tex b/tex/thesis.tex index b0af664..85a8294 100644 --- a/tex/thesis.tex +++ b/tex/thesis.tex @@ -1197,7 +1197,7 @@ used this in subsequent processes. binding curve of \subcap{fig:biotin_coating}{biotin}, \subcap{fig:stp_coating}{\gls{stp}}, and \subcap{fig:mab_coating}{\glspl{mab}} as a function of biotin added. } - \label{fig:dms_flowchart} + \label{fig:dms_coating} \end{figure*} % TODO these caption titles suck @@ -1639,7 +1639,7 @@ bead over \SI{14}{\day} and had similar viability (\cref{fig:grex_results_fc,fig:grex_results_viability}). Furthermore, consistent with past results, \glspl{dms}-expanded T cells had higher \pthp{} compared to beads, but only had slightly higher \ptmemp{} compared to beads -(\cref{fig:grex_phenotype}). +(\cref{fig:grex_mem,fig:grex_cd4}). % TODO is this discussion stuff? These discrepancies might be explained in light of our other data as follows. @@ -2957,6 +2957,7 @@ them to grow better in the \gls{dms} system. \begingroup \includegraphics{../figures/integrin_1.png} + \phantomsubcaption\label{fig:inegrin_1_overview} \phantomsubcaption\label{fig:inegrin_1_fc} \phantomsubcaption\label{fig:inegrin_1_mem} \phantomsubcaption\label{fig:inegrin_1_cd49} @@ -2964,6 +2965,11 @@ them to grow better in the \gls{dms} system. \endgroup \caption[Integrin blocking I] {Blocking with integrin does not lead to differences in memory or growth. + \subcap{fig:inegrin_1_overview}{Experimental overview} + \subcap{fig:inegrin_1_fc}{Fold change of \gls{dms}-activated T cell over + time with each blocking condition.} + \subcap{fig:inegrin_1_mem}{\ptmemp{} at day 14 for each blocked condition.} + \subcap{fig:inegrin_1_cd49}{\gls{a2b1} and \gls{a2b2} expression over time.} } \label{fig:integrin_1} \end{figure*} @@ -2986,12 +2992,16 @@ of the groups (\cref{fig:inegrin_1_mem}). We also noted that \gls{a2b1} and \begingroup \includegraphics{../figures/integrin_2.png} + \phantomsubcaption\label{fig:inegrin_2_overview} \phantomsubcaption\label{fig:inegrin_2_fc} \phantomsubcaption\label{fig:inegrin_2_mem} \endgroup \caption[Integrin blocking II] {Blocking with integrin does not lead to differences in memory or growth. + \subcap{fig:inegrin_1_fc}{Fold change of \gls{dms}-activated T cell over + time with each blocking condition.} + \subcap{fig:inegrin_1_mem}{\ptmemp{} at day 14 for each blocked condition.} } \label{fig:integrin_2} \end{figure*}