ENH split Cd19 car fig

tex/thesis.tex

@@ -1937,7 +1937,6 @@ appeared that the \gls{mab} binding was quadratically related to biotin binding
 critical to controlling the amount and \glspl{mab} and thus the amount of signal
 the T cells receive downstream.
 
-% FIGURE combine this DOE figure into one interaction plot
 \begin{figure*}[ht!]
   \begingroup
 
@@ -2367,7 +2366,7 @@ generate \gls{car} T cells\cite{Tumaini2013, Lamers2014}. We added a
 \SI{24}{\hour} transduction step on day 1 of the \SI{14}{\day} expansion to
 insert an anti-CD19 \gls{car}\cite{Milone2009} and subsequently measured the
 surface expression of the \gls{car} on day 14
-(\cref{fig:car_production_flow_pl,fig:car_production_endpoint_pl}). We noted
+(\cref{fig:car_cd19_flow,fig:car_cd19_endpoint}). We noted
 that there was robust \gls{car} expression in over \SI{25}{\percent} of expanded
 T cells, and there was no observable difference in \gls{car} expression between
 beads and \glspl{dms}.
@@ -2380,7 +2379,7 @@ appearance of CD107a on CD8+ T cells. CD107a is found on the inner-surface of
 cytotoxic granules and will emerge on the surface after cytotoxic T cells are
 activated and degranulate. Indeed, we observed degranulation in T cells expanded
 with both beads and \glspl{dms}, although not to an observably different degree
-(\cref{fig:car_production_flow_degran,fig:car_production_endpoint_degran}).
+(\cref{fig:car_degran_flow,fig:car_degran_endpoint}).
 Taken together, these results indicated that the \glspl{dms} provide similar
 transduction efficiency compared to beads.
 
@@ -2389,7 +2388,7 @@ for CCL21; since \glspl{dms} produced a larger percentage of naïve and memory T
 cells (which have CCR7, the receptor for CCL21) we would expect higher migration
 in \gls{dms}-expanded cells vs.\ their bead counterparts. Indeed, we noted a
 significantly higher migration percentage for T cells grown using \glspl{dms}
-versus beads (\cref{fig:car_production_migration}). Interestingly, there also
+versus beads (\cref{fig:car_degran_migration}). Interestingly, there also
 appeared to be a decrease in CCL21 migration between transduced and untransduced
 T cells expanded using beads, but this interaction effect was only weakly
 significant (p = 0.068). No such effect was seen for \gls{dms}-expanded T cells,
@@ -2399,25 +2398,38 @@ showing that migration was likely independent of \gls{car} transduction.
 \begin{figure*}[ht!]
   \begingroup
 
-  \includegraphics{../figures/car_production.png}
+  \includegraphics{../figures/car_cd19.png}
-  \phantomsubcaption\label{fig:car_production_flow_pl}
+  \phantomsubcaption\label{fig:car_cd19_flow}
-  \phantomsubcaption\label{fig:car_production_endpoint_pl}
+  \phantomsubcaption\label{fig:car_cd19_endpoint}
-  \phantomsubcaption\label{fig:car_production_flow_degran}
+
-  \phantomsubcaption\label{fig:car_production_endpoint_degran}
+  \endgroup
-  \phantomsubcaption\label{fig:car_production_migration}
+  \caption[\glspl{dms} lead to efficient CD19 transduction]
+  {\glspl{dms} lead to efficient CD19 transduction.
+    \subcap{fig:car_cd19_flow}{Representative flow cytometry plot for
+      transduced or untransduced T cells stained with \gls{ptnl}.}
+    \subcap{fig:car_cd19_endpoint}{Endpoint plots with \gls{anova} for
+      transduced or untransduced T cells stained with \gls{ptnl}.}
+    All data is from T cells expanded for \SI{14}{\day}.
+  }
+  \label{fig:car_production}
+\end{figure*}
+
+\begin{figure*}[ht!]
+  \begingroup
+
+  \includegraphics{../figures/car_degranulation.png}
+  \phantomsubcaption\label{fig:car_degran_flow}
+  \phantomsubcaption\label{fig:car_degran_endpoint}
+  \phantomsubcaption\label{fig:car_degran_migration}
 
   \endgroup
   \caption[\glspl{dms} produce functional \gls{car} T cells]
   {\glspl{dms} produce functional \gls{car} T cells.
-    \subcap{fig:car_production_flow_pl}{Representative flow cytometry plot for
+    \subcap{fig:car_degran_flow}{Representative flow plot for
-      transduced or untransduced T cells stained with \gls{ptnl}.}
-    \subcap{fig:car_production_endpoint_pl}{Endpoint plots with \gls{anova} for
-      transduced or untransduced T cells stained with \gls{ptnl}.}
-    \subcap{fig:car_production_flow_degran}{Representative flow plot for
       degenerating T cells.}
-    \subcap{fig:car_production_endpoint_degran}{Endpoint plots for transduced or
+    \subcap{fig:car_degran_endpoint}{Endpoint plots for transduced or
     untransduced T cells stained with \cd{107a} for the degranulation assay.}
-    \subcap{fig:car_production_migration}{Endpoint plot for transmigration assay
+    \subcap{fig:car_degran_migration}{Endpoint plot for transmigration assay
     with \gls{anova}.} All data is from T cells expanded for \SI{14}{\day}.
   }
   \label{fig:car_production}