ADD method for luminex

tex/thesis.tex

@@ -931,9 +931,22 @@ plates and incubated for another \SI{24}{\hour}. Cells and beads/\glspl{dms}
 were removed from the retronectin plates using vigorous pipetting and
 transferred to another 96 well plate wherein expansion continued.
 
-\subsection{Luminex}
+\subsection{Luminex Analysis}
 
-% METHOD luminex
+Luminex was performed using a \product{ProcartaPlex kit}{\thermo}{custom} for
+the markers outlined in \cref{tab:luminex_panel} with modifications (note that
+some markers were run in separate panels to allow for proper dilutions).
+Briefly, media supernatents from cells were sampled as desired and immediately
+placed in a \SI{-80}{\degreeCelsius} freezer until use. Before use, samples were
+thawed at \gls{rt} and vortexed to ensure homogeneity. To run the plate,
+\SI{25}{\ul} of magnetic beads were added to the plate and washed 3x using
+\SI{300}{\ul} of wash buffer. \SI{25}{\ul} of samples or standard were added to
+the plate and incubated for \SI{120}{\minute} at \SI{850}{\rpm} at \gls{rt}
+before washing analogously 3X with wash. \SI{12.5}{\ul} detection \glspl{mab}
+and \SI{25}{\ul} \gls{stppe} were sequentially added, incubated for
+\SI{30}{\minute} and vortexed, and washed analogously to the sample step.
+Finally, samples were resuspended in \SI{120}{\ul} reading buffer and analyzed
+via a Biorad Bioplex 200 plate reader.
 
 \begin{table}[!h] \centering
   \caption{Luminex Panel}