ADD method for luminex
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@ -931,9 +931,22 @@ plates and incubated for another \SI{24}{\hour}. Cells and beads/\glspl{dms}
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were removed from the retronectin plates using vigorous pipetting and
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transferred to another 96 well plate wherein expansion continued.
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\subsection{Luminex}
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\subsection{Luminex Analysis}
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% METHOD luminex
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Luminex was performed using a \product{ProcartaPlex kit}{\thermo}{custom} for
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the markers outlined in \cref{tab:luminex_panel} with modifications (note that
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some markers were run in separate panels to allow for proper dilutions).
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Briefly, media supernatents from cells were sampled as desired and immediately
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placed in a \SI{-80}{\degreeCelsius} freezer until use. Before use, samples were
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thawed at \gls{rt} and vortexed to ensure homogeneity. To run the plate,
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\SI{25}{\ul} of magnetic beads were added to the plate and washed 3x using
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\SI{300}{\ul} of wash buffer. \SI{25}{\ul} of samples or standard were added to
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the plate and incubated for \SI{120}{\minute} at \SI{850}{\rpm} at \gls{rt}
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before washing analogously 3X with wash. \SI{12.5}{\ul} detection \glspl{mab}
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and \SI{25}{\ul} \gls{stppe} were sequentially added, incubated for
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\SI{30}{\minute} and vortexed, and washed analogously to the sample step.
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Finally, samples were resuspended in \SI{120}{\ul} reading buffer and analyzed
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via a Biorad Bioplex 200 plate reader.
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\begin{table}[!h] \centering
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\caption{Luminex Panel}
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