diff --git a/figures/il15_blockade_1.svg b/figures/il15_blockade_1.svg
index 2d3628a..10d9a5a 100644
--- a/figures/il15_blockade_1.svg
+++ b/figures/il15_blockade_1.svg
@@ -445,6 +445,538 @@
d="m 213.29,204.14 h 74.37 v 17.15 h -74.37 z"
id="path33588" />
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+ bead
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+ DMS
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+ 0
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+ 10
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+ 0
+ 5
+ 10
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+ 0%
+ 25%
+ 50%
+ 75%
+ 100%
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+ Viability
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+ IL15Ra Block
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+ TRUE
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+ transform="matrix(1.3333333,0,0,-1.3333333,7.066683,620.18669)">
-
-
fold change
- viability
- a.
+ x="0.72211659"
+ y="136">b.
a.
+ b.
+ x="286.00003"
+ y="136">c.
c.
+ x="1.2924291"
+ y="318.00003">d.
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+ bead
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+ DMS
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+ 10
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+ 0
+ 5
+ 10
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+ 0.0e+00
+ 5.0e+06
+ 1.0e+07
+ 1.5e+07
+ 2.0e+07
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+ Time (days)
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+ Number of cells
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+ IL15Ra Block
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+ FALSE
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+ TRUE
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+
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+ D0
+ D6
+ D4
+ D2
+ D14
+ Activate
+ Harvest
+
+
+
+ Add anti-IL15Ror isotype control(5mg/ml)
diff --git a/figures/il15_blockade_2.svg b/figures/il15_blockade_2.svg
index a46bdb3..2eddf8f 100644
--- a/figures/il15_blockade_2.svg
+++ b/figures/il15_blockade_2.svg
@@ -298,6 +298,104 @@
d="m 212.85,205.02 h 74.81 v 16.27 h -74.81 z"
id="path44124" />
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+ transform="matrix(1.3333333,0,0,-1.3333333,9.293345,592.96002)">
-
-
a.
+ x="0.26512516"
+ y="125.30667">b.
a.
+ b.
+ x="210.61337"
+ y="125.30667">c.
c.
+ x="0.83543766"
+ y="292">d.
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+ 0e+00
+ 2e+08
+ 4e+08
+ 6e+08
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+ 0
+ 5
+ 10
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+ Time (days)
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+ Number of cells
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+
+
+ IL15 Block
+
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+ FALSE
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+ TRUE
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+ 0%
+ 25%
+ 50%
+ 75%
+ 100%
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+ 0
+ 5
+ 10
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+ Time (days)
+
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+ Viability
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+
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+
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+ IL15 Block
+
+
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+
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+
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+ FALSE
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+ TRUE
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+
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+
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+
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+
+
+
+
fold change
+ id="tspan111335"
+ x="30.248056"
+ y="32.02758">D0
viability
+ id="tspan111335-2"
+ x="102.23439"
+ y="32.02758">D6
+ D8
+ D4
+ D2
+ D14
+ Activate
+ Harvest
+
+
+
+
+ Add anti-IL15or isotype control(5mg/ml)
diff --git a/tex/thesis.tex b/tex/thesis.tex
index 85a8294..a581bbd 100644
--- a/tex/thesis.tex
+++ b/tex/thesis.tex
@@ -3033,27 +3033,31 @@ many conditions, we hypothesized that \gls{il15} may be responsible for this,
and further that the unique \textit{cis/trans} activity of \gls{il15} may be
more active in the \gls{dms} system due to higher cell density.
-% TODO actually add captions
-% TODO add fold change and viability to these
-% TODO maybe combine these
% TODO add some background into why IL15 is important and the proposed mechanism
\begin{figure*}[ht!]
\begingroup
\includegraphics{../figures/il15_blockade_1.png}
+ \phantomsubcaption\label{fig:il15_1_overview}
\phantomsubcaption\label{fig:il15_1_fc}
\phantomsubcaption\label{fig:il15_1_viability}
\phantomsubcaption\label{fig:il15_1_mem}
\endgroup
\caption[IL15 blocking I]
- {Blocking with IL15 does not lead to differences in memory or growth.
+ {Blocking IL15Ra does not lead to differences in memory or growth.
+ \subcap{fig:il15_1_overview}{Experimental overview}
+ Longitudinal measurements of
+ \subcap{fig:il15_1_fc}{fold change} and
+ \subcap{fig:il15_1_viability}{viability} for blocked and unblocked
+ conditions expanded with either beads or \glspl{dms}.
+ \subcap{fig:il15_1_mem}{Flow cytometry markers for \gls{dms}-expanded T
+ cells at day 14 for blocked and unblocked groups.}.
}
\label{fig:il15_1}
\end{figure*}
% TODO how did I determine how much to add?
-% TODO how often was this added?
% TODO just gate these as normal because this looks sketchy
We first tested this hypothesis by blocking \gls{il15r} with either a specific
\gls{mab} or an \gls{igg} isotype control. We observed no difference in the
@@ -3070,13 +3074,21 @@ the markers, and by extension showing no difference in phenotype
\begingroup
\includegraphics{../figures/il15_blockade_2.png}
+ \phantomsubcaption\label{fig:il15_2_overview}
\phantomsubcaption\label{fig:il15_2_fc}
\phantomsubcaption\label{fig:il15_2_viability}
\phantomsubcaption\label{fig:il15_2_mem}
\endgroup
\caption[IL15 blocking II]
- {Blocking with IL15 does not lead to differences in memory or growth.
+ {Blocking soluble IL15 does not lead to differences in memory or growth.
+ \subcap{fig:il15_2_overview}{Experimental overview}
+ Longitudinal measurements of
+ \subcap{fig:il15_2_fc}{fold change} and
+ \subcap{fig:il15_2_viability}{viability} for blocked and unblocked
+ conditions expanded with \glspl{dms}.
+ \subcap{fig:il15_2_mem}{Flow cytometry markers for \gls{dms}-expanded T
+ cells at day 14 for blocked and unblocked groups.}.
}
\label{fig:il15_2}
\end{figure*}