From c610ddb40e94d7eaff1f40fe5c9378bcb6ce9fe3 Mon Sep 17 00:00:00 2001
From: ndwarshuis <ndwar@yavin4.ch>
Date: Tue, 27 Jul 2021 13:05:01 -0400
Subject: [PATCH] ADD discussion to aim 3 mouse 1

---
 tex/thesis.tex | 20 ++++++++++++++++++--
 1 file changed, 18 insertions(+), 2 deletions(-)

diff --git a/tex/thesis.tex b/tex/thesis.tex
index bdfa172..4873e30 100644
--- a/tex/thesis.tex
+++ b/tex/thesis.tex
@@ -157,6 +157,7 @@
 \newcommand{\ptmemh}{\pth\ptmem}
 \newcommand{\ptmemk}{\ptk\ptmem}
 \newcommand{\dpthp}{$\Updelta$\pthp{}}
+\newcommand{\carp}{\gls{car}+~\si{\percent}}
 
 \newcommand{\catnum}[2]{(#1, #2)}
 \newcommand{\product}[3]{#1 \catnum{#2}{#3}}
@@ -2072,8 +2073,7 @@ cells are effective at lower doses.
     \subcap{fig:mouse_timecourse_qc_growth}{Fold change of T cells (each
       timepoint only includes the runs that were harvested at day 14).}
     Fractions of T cell subtypes in the day 14 product including
-    % TODO refactor CAR+ percent into nice macro
-    \subcap{fig:mouse_timecourse_qc_car}{\gls{car}+~\si{\percent}},
+    \subcap{fig:mouse_timecourse_qc_car}{\carp{}},
     \subcap{fig:mouse_timecourse_qc_cd4}{\pthp{}}, and
     \subcap{fig:mouse_timecourse_qc_mem}{\ptmemp{}}.
   }
@@ -2103,6 +2103,22 @@ cells are effective at lower doses.
 
 \section{discussion}
 
+When we tested bead and DMS expanded \gls{car} T cells, we also found that the
+\gls{dms} expanded CAR-T cells outperformed bead groups in prolonging survival
+of Nalm-6 tumor challenged (intravenously injected) \gls{nsg} mice. DMS expanded
+CAR-T cells were very effective in clearing tumor cells as early as 7 days post
+CAR-T injection even at low total T cell dose compared to the bead groups where
+tumor burden was higher than DMS groups across all the total T cell doses tested
+here. More interestingly, when only CAR-expressing T cell doses between bead and
+DMS groups were compared, DMS group had significantly higher survival effects
+over similar or higher CAR expression T cell doses from bead group. All these
+results suggest that the higher proportion of memory T cells in DMS groups
+(compared to bead group) resulted in highly effective CAR-T cells that can
+efficiently kill tumor cells as recently reported in
+literature\cite{Fraietta2018, Sommermeyer2015}.
+
+% DISCUSSION 2nd mouse model
+
 \chapter{conclusions and future work}\label{conclusions}
 \section{conclusions}
 \section{future work}