diff --git a/tex/thesis.tex b/tex/thesis.tex index b230661..926dffb 100644 --- a/tex/thesis.tex +++ b/tex/thesis.tex @@ -698,6 +698,7 @@ Therefore, the proposed microcarrier system should enable greater expansion and better retention of memory phenotype compared to current bead-based methods. \section{methods} + \subsection{dms functionalization} \begin{figure*}[ht!] @@ -834,6 +835,8 @@ Cells on the \glspl{dms} were visualized by adding \SI{0.5}{\ul} \product{\acd{45}-\gls{af647}}{\bl}{368538}, incubating for \SI{1}{\hour}, and imaging on a spinning disk confocal microscope. +% METHOD mtt staining and interior quantification + \subsection{quantification of apoptosis using Annexin-V} Apoptosis was quantified using \gls{anv} according to the manufacturer's @@ -905,6 +908,7 @@ Briefly, cells were washed once and stained with \product{biotinylated BD Accuri. Readout was percent \gls{pe}+ cells as compared to secondary controls (\gls{pe}-\gls{stp} with no \gls{ptnl}). +% TODO add BCMA-CAR stuff \subsection{car plasmid and lentiviral transduction} The anti-CD19-CD8-CD137-CD3z \gls{car} with the EF1$\upalpha$ @@ -924,8 +928,6 @@ plates and incubated for another \SI{24}{\hour}. Cells and beads/\glspl{dms} were removed from the retronectin plates using vigorous pipetting and transferred to another 96 well plate wherein expansion continued. - - \subsection{statistical analysis} For 1-way \gls{anova} analysis with Tukey multiple comparisons test, @@ -947,7 +949,8 @@ lack-of-fit tests where replicates were present (to assess model fit in the context of pure error). Statistical significance was evaluated at $\upalpha$ = 0.05. -% TODO add meta-analysis section +% METHOD add meta-analysis +% METHOD add Grex \section{results} @@ -976,9 +979,8 @@ noted that the maximal \gls{mab} binding capacity occurred near \SI{50}{\nmol} biotin input (which corresponded to \SI{2.5}{\nmol\per\mg\of{\dms}}) thus we used this in subsequent processes. -% TODO add paragraph explaining the qc stuff - -% TODO add paragraph explaining the reaction kinetics stuff +% RESULT add paragraph explaining the qc stuff +% RESULT add paragraph explaining the reaction kinetics stuff % TODO flip the rows of this figure (right now the text is backward) \begin{figure*}[ht!] @@ -1135,7 +1137,6 @@ cells after \SI{14}{\day} were on the interior surface of the \glspl{dms} \label{fig:dms_flowchart} \end{figure*} -% TODO add this to the methods section % TODO double check the timing of this experiment (it might not be day 14) % TODO provide the regression results and coefficients from this \begin{figure*}[ht!] @@ -1290,9 +1291,17 @@ showing that migration was likely independent of \gls{car} transduction. \subcap{fig:car_production_migration}{Endpoint plot for transmigration assay with \gls{anova}.} All data is from T cells expanded for \SI{14}{\day}. } - \label{fig:dms_phenotype} + \label{fig:car_production} \end{figure*} +% FIGURE bcma results + +\subsection{DMSs efficiently expand T cells in Grex bioreactors} + +% FIGURE grex +% FIGURE luminex +% FIGURE grex + car + \subsection{DMSs do not leave antibodies attached to cell product} We asked if \glspl{mab} from the \glspl{dms} detached from the \gls{dms} surface @@ -1625,6 +1634,8 @@ provide these benefits. \subsection{DOE shows optimal conditions for expanded potent T cells} +% TABLE luminex panel used + % TODO this plots proportions look dumb \begin{figure*}[ht!] \begingroup @@ -1699,7 +1710,7 @@ provide these benefits. \label{fig:doe_responses} \end{figure*} -% TODO add DOE regression tables +% TABLE DOE regression results % TODO this section header sucks \subsection{AI modeling reveals highly predictive species}