From e93859056deb18d8cd528de6909d916250796950 Mon Sep 17 00:00:00 2001 From: ndwarshuis Date: Tue, 27 Jul 2021 13:20:35 -0400 Subject: [PATCH] ADD full survival curve to first mouse experiment --- figures/mouse_dosing_ivis.svg | 3929 +++++++++++++++++---------------- tex/thesis.tex | 23 +- 2 files changed, 2004 insertions(+), 1948 deletions(-) diff --git a/figures/mouse_dosing_ivis.svg b/figures/mouse_dosing_ivis.svg index 750d84b..e5eac79 100644 --- a/figures/mouse_dosing_ivis.svg +++ b/figures/mouse_dosing_ivis.svg @@ -1000,6 +1000,18 @@ id="path1058" d="M 361.85937,636 H 410 v 73 h -48.14063 z m 0,0" /> + + + + inkscape:pagecheckerboard="0" + inkscape:snap-grids="false"> + transform="matrix(0.67842202,0,0,0.67842202,-15.067948,-101.23376)"> - - - - - - - - - - - - - 15 - 20 - 25 - 30 - 35 - 40 - 45 - 50 - - - - - - - - - - 0 - 20 - 40 - 60 - 80 - 100 - - - - - - - - - - - Days elapsed - - - Percent survival - - Untreated - - - Bead MedDose - - - - Bead LoDose - - - - Bead HiDose - - - - DMS HiDose - - - - DMS MedDose - - - - DMS LoDose - - - - - - ** - Bead - Untreated - DMS - - - - - ** - - - - - ** - - - - - * - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - + id="tspan107620" /> b. + y="277.97845">b. c. + x="281.20248" + y="277.97845">c. d. + x="0.61781627" + y="443.32932">d. e. + + + + + + + + + + + + + + 15 + 20 + 25 + 30 + 35 + 40 + 45 + 50 + + + + + + + + + + 0 + 20 + 40 + 60 + 80 + 100 + + + + + + + + + + + Days elapsed + + + Percent survival + + Untreated + + + Bead MedDose + + + + Bead LoDose + + + + Bead HiDose + + + + DMS HiDose + + + + DMS MedDose + + + + DMS LoDose + + + + + + ** + Bead + Untreated + DMS + + + + + ** + + + + + ** + + + + + * + + + + Survial + y="425.57568" + id="text121981">Survial + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + Survival (Bead High or Med vs DMS Lo) + Survival (Bead High or Med vs DMS Lo) + id="tspan121983-6" + x="357.25656" + y="451.4642">Survival (up to day 46) + diff --git a/tex/thesis.tex b/tex/thesis.tex index 4873e30..b478e89 100644 --- a/tex/thesis.tex +++ b/tex/thesis.tex @@ -1960,15 +1960,16 @@ of both the bead medium dose and the bead high dose of Nalm-6 tumor bearing \gls{nsg} mice shown in the \cref{fig:mouse_dosing_ivis_survival} was up to day 40 as reported elsewhere\cite{Fraietta2018}. However, we also included a Kaplan-Meier figure up -to day 46 (Supplemental Figure 7) where most of the mice euthanized from day 40 -through day 46 from \gls{dms} groups showed no or very small fragment of spleen -which was due to \gls{gvhd} responses. Similar \gls{gvhd} responses were -reported earlier in \gls{nsg} mice where the mice injected with human \gls{pbmc} -exhibited acute \gls{gvhd} between \SIrange{40}{50}{\day} post intravenous -injection\cite{Ali2012}. Notably, both survival analyses (up to day 40 in -\cref{fig:mouse_dosing_ivis_survival} and up to day 46 in Supplemental Figure 7) -confirmed that \gls{dms}-expanded groups outperformed bead-expanded groups in -terms of prolonging survival of Nalm-6 tumor challenged \gls{nsg} mice. +to day 46 (\cref{fig:mouse_dosing_ivis_survival_full}) where most of the mice +euthanized from day 40 through day 46 from \gls{dms} groups showed no or very +small fragment of spleen which was due to \gls{gvhd} responses. Similar +\gls{gvhd} responses were reported earlier in \gls{nsg} mice where the mice +injected with human \gls{pbmc} exhibited acute \gls{gvhd} between +\SIrange{40}{50}{\day} post intravenous injection\cite{Ali2012}. Notably, both +survival analyses (up to day 40 in \cref{fig:mouse_dosing_ivis_survival} and up +to day 46 in \cref{fig:mouse_dosing_ivis_survival_full}) confirmed that +\gls{dms}-expanded groups outperformed bead-expanded groups in terms of +prolonging survival of Nalm-6 tumor challenged \gls{nsg} mice. Together, these data suggested that \glspl{dms} produce T cells that are not only more potent that bead-expanded T cells (even when accounting for @@ -2026,6 +2027,7 @@ cells are effective at lower doses. \phantomsubcaption\label{fig:mouse_dosing_ivis_plots} \phantomsubcaption\label{fig:mouse_dosing_ivis_survival} \phantomsubcaption\label{fig:mouse_dosing_ivis_survival_comp} + \phantomsubcaption\label{fig:mouse_dosing_ivis_survival_full} \endgroup \caption[Mouse Dosing IVIS and Survival Results] @@ -2038,6 +2040,9 @@ cells are effective at lower doses. \subcap{fig:mouse_dosing_ivis_survival}{Survival plots of mice} \subcap{fig:mouse_dosing_ivis_survival_comp}{Survival plots of mice showing only those that received a comparable number of \gls{car} T cells.} + \subcap{fig:mouse_dosing_ivis_survival_full}{The same data as + \subref{fig:mouse_dosing_ivis_survival} except showing the full time until + euthanasia for all mice (including those that died via \gls{gvhd}).} } \label{fig:mouse_dosing_ivis} \end{figure*}