diff --git a/tex/thesis.tex b/tex/thesis.tex index 974ba9e..5fbc221 100644 --- a/tex/thesis.tex +++ b/tex/thesis.tex @@ -2095,18 +2095,18 @@ Flow cytometry was performed analogously to \cref{sec:flow_cytometry}. Cytokines were quantified via Luminex as described in \cref{sec:luminex_analysis}. -% TODO add a footnote saying I didn't do this \subsection{NMR metabolomics} Prior to analysis, samples were centrifuged at \SI{2990}{\gforce} for -\SI{20}{\minute} at \SI{4}{\degreeCelsius} to clear any debris. 5 uL of 100/3 mM -DSS-D6 in deuterium oxide (Cambridge Isotope Laboratories) were added to 1.7 mm -NMR tubes (Bruker BioSpin), followed by 45 uL of media from each sample that was -added and mixed, for a final volume of 50 uL in each tube. Samples were prepared -on ice and in predetermined, randomized order. The remaining volume from each -sample in the rack (approx. 4 uL) was combined to create an internal pool. This -material was used for internal controls within each rack as well as metabolite -annotation. +\SI{20}{\minute} at \SI{4}{\degreeCelsius} to clear any debris\footnote{all + \gls{nmr} analysis was done by our collaborators at the University of + Georgia}. 5 uL of 100/3 mM DSS-D6 in deuterium oxide (Cambridge Isotope +Laboratories) were added to 1.7 mm NMR tubes (Bruker BioSpin), followed by 45 uL +of media from each sample that was added and mixed, for a final volume of 50 uL +in each tube. Samples were prepared on ice and in predetermined, randomized +order. The remaining volume from each sample in the rack (approx. 4 uL) was +combined to create an internal pool. This material was used for internal +controls within each rack as well as metabolite annotation. \gls{nmr} spectra were collected on a Bruker Avance III HD spectrometer at 600 MHz using a 5-mm TXI cryogenic probe and TopSpin software (Bruker BioSpin).