From ed63f137974d71cf4e52fe7a68fbf2ca183431a4 Mon Sep 17 00:00:00 2001 From: ndwarshuis Date: Tue, 27 Jul 2021 11:07:28 -0400 Subject: [PATCH] ADD apoptosis figure --- figures/apoptosis.svg | 1359 +++++++++++++++++++++++++++++++++++++++++ tex/thesis.tex | 27 +- 2 files changed, 1385 insertions(+), 1 deletion(-) create mode 100644 figures/apoptosis.svg diff --git a/figures/apoptosis.svg b/figures/apoptosis.svg new file mode 100644 index 0000000..5036a9f --- /dev/null +++ b/figures/apoptosis.svg @@ -0,0 +1,1359 @@ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + image/svg+xml + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + T−test, p = 0.012 + + + + + + + + + + + + + T−test, p = 0.015 + + + + + + + + + + apoptotic + + + + + + + + + + + + + + + + necrotic + + + + + + + + + + + + + + + + + + + + + + + + + + bead + DMS + + + + + + + + + + + + + + + + + bead + DMS + + + + + + + + + + + + + + 0.0% + 10.0% + 20.0% + 30.0% + 40.0% + 50.0% + + + + + + + + + + + + + + + + + + + + + + + Percent of all T cells + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + T−test, p = 0.011 + + + + + + + + + + + + + + + + + + + + + 30.0% + 40.0% + 50.0% + 60.0% + 70.0% + + + + + + + + + + + + + + + + + + + + + + + + + + + bead + DMS + + + + + + + + + + Percent of all T cells + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 2000 + 3000 + 4000 + 5000 + 6000 + 7000 + + + + + + + + + + + + + + + + + + + + + + + + + + + + bead + DMS + + + + + + + + + + BCL−2 Concentration (ug/ml) + + + + + + + + + + + + + + + a. + b. + c. + + diff --git a/tex/thesis.tex b/tex/thesis.tex index 60b2030..9eedefc 100644 --- a/tex/thesis.tex +++ b/tex/thesis.tex @@ -88,6 +88,8 @@ \newacronym{colb}{COL-B}{collagenase B} \newacronym{cold}{COL-D}{collagenase D} \newacronym{tsne}{tSNE}{t-stochastic neighbor embedding} +\newacronym{anv}{ANX-V}{Annexin-V} +\newacronym{pi}{PI}{propidium iodide} %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% % SI units for uber nerds @@ -880,6 +882,8 @@ plates and incubated for another \SI{24}{\hour}. Cells and beads/\glspl{dms} were removed from the retronectin plates using vigorous pipetting and transferred to another 96 well plate wherein expansion continued. +% METHOD for apoptosis quantification + \subsection{statistical analysis} For 1-way \gls{anova} analysis with Tukey multiple comparisons test, @@ -1066,7 +1070,28 @@ cells after \SI{14}{\day} were on the interior surface of the \glspl{dms} %, and this did not significantly change with initial seeding density (Supplemental Table 1). -% FIGURE caspase/bcl2 figure +\begin{figure*}[ht!] + \begingroup + + \includegraphics{../figures/apoptosis.png} + \phantomsubcaption\label{fig:apoptosis_annV} + \phantomsubcaption\label{fig:apoptosis_cas} + \phantomsubcaption\label{fig:apoptosis_bcl2} + + \endgroup + \caption[Apoptosis Quantification for \glspl{dms}] + {\glspl{dms} produce cells with lower apoptosis marker expression on average + compared to bead. + \subcap{fig:apoptosis_annV}{Quantification of apoptosis and necrosis by + \gls{anv} and \gls{pi}.} + \subcap{fig:apoptosis_cas}{Quantification of Caspase-3/7 expression using + CellEvent dye.} + \subcap{fig:apoptosis_bcl2}{Quantification of BCL-2 expression using + \gls{elisa}. All statistical tests shown are two-tailed homoschodastic + t-tests.} + } + \label{fig:dms_flowchart} +\end{figure*} % TODO add this to the methods section % TODO double check the timing of this experiment (it might not be day 14)