ADD results paragraph for grex
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@ -1466,7 +1466,25 @@ for bead (\cref{fig:car_bcma_total}).
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\subsection{DMSs efficiently expand T cells in Grex bioreactors}
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\subsection{DMSs efficiently expand T cells in Grex bioreactors}
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% RESULT Grex bioreactor and luminex
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We also asked if the \gls{dms} platform could expand T cells in a static
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bioreactor such a Grex. We incubated T cells in a Grex analogously to that for
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plates and found that T cells in Grex bioreactors expanded as efficiently as
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bead over \SI{14}{\day} and had similar viability
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(\cref{fig:grex_results_fc,fig:grex_results_viability}). Furthermore, consistent
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with past results, \glspl{dms}-expanded T cells had higher \pthp{} compared to
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beads, but only had slightly higher \ptmemp{} compared to beads
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(\cref{fig:grex_phenotype}).
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% TODO is this discussion stuff?
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These discrepancies might be explained in light of our other data as follows.
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The Grex bioreactor has higher media capacity relative to its surface area, and
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we did not move the T cells to a larger bioreactor as they grew in contrast with
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our plate cultures. This means that the cells had higher growth area
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constraints, which may have nullified any advantage to the expansion that we
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seen elsewhere (\cref{fig:dms_exp_fold_change}). Furthermore, the higher growth
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area could mean higher signaling and higher differentiation rate to effector T
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cells, which was why the \ptmemp{} was so low compared to other data
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(\cref{fig:dms_phenotype_mem}).
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% TODO this figure has wonky proportions
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% TODO this figure has wonky proportions
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% TODO add stats for the phenotype stuff
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% TODO add stats for the phenotype stuff
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