ADD results paragraph for grex
This commit is contained in:
parent
67a4758da0
commit
eda1f3e6d6
|
@ -1466,7 +1466,25 @@ for bead (\cref{fig:car_bcma_total}).
|
|||
|
||||
\subsection{DMSs efficiently expand T cells in Grex bioreactors}
|
||||
|
||||
% RESULT Grex bioreactor and luminex
|
||||
We also asked if the \gls{dms} platform could expand T cells in a static
|
||||
bioreactor such a Grex. We incubated T cells in a Grex analogously to that for
|
||||
plates and found that T cells in Grex bioreactors expanded as efficiently as
|
||||
bead over \SI{14}{\day} and had similar viability
|
||||
(\cref{fig:grex_results_fc,fig:grex_results_viability}). Furthermore, consistent
|
||||
with past results, \glspl{dms}-expanded T cells had higher \pthp{} compared to
|
||||
beads, but only had slightly higher \ptmemp{} compared to beads
|
||||
(\cref{fig:grex_phenotype}).
|
||||
|
||||
% TODO is this discussion stuff?
|
||||
These discrepancies might be explained in light of our other data as follows.
|
||||
The Grex bioreactor has higher media capacity relative to its surface area, and
|
||||
we did not move the T cells to a larger bioreactor as they grew in contrast with
|
||||
our plate cultures. This means that the cells had higher growth area
|
||||
constraints, which may have nullified any advantage to the expansion that we
|
||||
seen elsewhere (\cref{fig:dms_exp_fold_change}). Furthermore, the higher growth
|
||||
area could mean higher signaling and higher differentiation rate to effector T
|
||||
cells, which was why the \ptmemp{} was so low compared to other data
|
||||
(\cref{fig:dms_phenotype_mem}).
|
||||
|
||||
% TODO this figure has wonky proportions
|
||||
% TODO add stats for the phenotype stuff
|
||||
|
|
Loading…
Reference in New Issue