ADD results section for il2 curves
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@ -2193,7 +2193,18 @@ Venn diagram from the venn R package.
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\subsection{T cells can be grown on DMSs with lower IL2 concentrations}
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\subsection{T cells can be grown on DMSs with lower IL2 concentrations}
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Prior to the main experiments in this aim, we performed a preliminary experiment
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to assess the effect of lowering the \gls{il2} concentration on the T cells
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grown with either bead or \gls{dms}. One of the hypotheses for the \gls{dms}
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system was that the higher cell density would enable more efficient cross-talk
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between T cells. Since \gls{il2} is secreted by activated T cells themselves,
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T cells in the \gls{dms} system may need less or no \gls{il2} if this hypothesis
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were true.
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% TODO this plots proportions look dumb
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% TODO this plots proportions look dumb
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% TODO explain what the NLS lines are in b
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% TODO plot the differences in lower IL2 concentrations to better show this
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\begin{figure*}[ht!]
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\begin{figure*}[ht!]
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\begingroup
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\begingroup
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@ -2217,6 +2228,29 @@ Venn diagram from the venn R package.
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\label{fig:il2_mod}
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\label{fig:il2_mod}
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\end{figure*}
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\end{figure*}
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% TODO the nls stuff is a bit iffy
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We varied the concentration of \gls{il2} from \SIrange{0}{100}{\IU\per\ml} and
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expanded T cells as described in \cref{sec:tcellculture}. T cells grown with
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either method expanded robustly as \gls{il2} concentration was increased
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(\cref{fig:il2_mod_timecourse}). Surprisingly, neither the bead or the \gls{dms}
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group expanded at all with \SI{0}{\IU\per\ml} \gls{il2}. When examining the
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endpoint fold change after \SI{14}{\day}, we observe that the difference between
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the bead and \gls{dms} appears to be greater at lower \gls{il2} concentrations
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(\cref{fig:il2_mod_total}). This is further supported by fitting a non-linear
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least squares equation to the data following a hyperbolic curve (which should be
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a plausible model given that this curve describes receptor-ligand kinetics,
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which we can assume \gls{il2} to follow). Furthermore, the same trend can be
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seen when only examining the \ptmem{} cell expansion at day 14
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(\cref{fig:il2_mod_mem}). In this case, the \ptmemp{} of the T cells seemed to
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be relatively close at higher \gls{il2} concentrations, but separated further at
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lower concentrations (\cref{fig:il2_mod_flow})
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Taken together, these data do not support the hypothesis that the \gls{dms}
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system does not need \gls{il2} at all; however, it appears to have a modest
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advantage at lower \gls{il2} concentrations compared to beads. For this reason,
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we decided to investigate the lower range of \gls{il2} concentrations starting
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at \SI{10}{\IU\per\ml} throughout the remainder of this aim.
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% TODO this is not consistent with the next section since the responses are
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% TODO this is not consistent with the next section since the responses are
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% different
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% different
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\subsection{DOE shows optimal conditions for expanded potent T cells}
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\subsection{DOE shows optimal conditions for expanded potent T cells}
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