ADD specific aims overview
This commit is contained in:
parent
8f6319c223
commit
fb98f0699b
|
@ -348,9 +348,52 @@ its effectiveness both \invivo{} and \invivo{}, and develop computational
|
|||
pipelines that could be used in a manufacturing environment.
|
||||
|
||||
\section*{specific aims}
|
||||
\subsection*{aim 1}
|
||||
\subsection*{aim 2}
|
||||
\subsection*{aim 3}
|
||||
|
||||
The specific aims of this dissertation are outlined in
|
||||
\cref{fig:graphical_overview}.
|
||||
|
||||
\begin{figure*}[ht!]
|
||||
\begingroup
|
||||
|
||||
\includegraphics[width=\textwidth]{example-image-a}
|
||||
|
||||
\endgroup
|
||||
\caption[Project Overview]{High-level workflow.}
|
||||
\label{fig:graphical_overview}
|
||||
\end{figure*}
|
||||
|
||||
\subsection*{aim 1: develop and optimize a novel T cell expansion process that
|
||||
mimics key components of the lymph nodes}
|
||||
|
||||
% TODO this might be easier to break apart in separate aims
|
||||
|
||||
In this first aim, we demonstrated the process for manufacturing \glspl{dms},
|
||||
including quality control steps that are necessary for translation of this
|
||||
platform into a scalable manufacturing setting. We also demonstrate that the
|
||||
\gls{dms} platform leads to higher overall expansion of T cells and higher
|
||||
overall fractions of potent memory and CD4+ subtypes desired for T cell
|
||||
therapies. Finally, we demonstrate \invitro{} that the \gls{dms} platform can be
|
||||
used to generate functional \gls{car} T cells targeted toward CD19.
|
||||
|
||||
\subsection*{aim 2: develop methods to control and predict T cell quality}
|
||||
|
||||
For this second aim, we investigated methods to identify and control \glspl{cqa}
|
||||
and glspl{cpp} for manufacturing T cells using the \gls{dms} platform. This was
|
||||
accomplished through two sub-aims:
|
||||
|
||||
\begin{itemize}
|
||||
\item[A --] Develop computational methods to control and predict T cell
|
||||
expansion and quality
|
||||
\item[B --] Perturb \gls{dms} expansion to identify additional mechanistic
|
||||
controls for expansion and quality
|
||||
\end{itemize}
|
||||
|
||||
\subsection*{aim 3: confirm potency of T cells from novel T cell expansion
|
||||
process using \invivo{} xenograft mouse model}
|
||||
|
||||
In this final aim, we demonstrate the effectiveness of \gls{dms}-expanded T
|
||||
cells compared to state-of-the-art beads using \invivo{} mouse models for
|
||||
\gls{all}.
|
||||
|
||||
\section*{outline}
|
||||
|
||||
|
|
Loading…
Reference in New Issue