ADD experimental overview and fold change/viability plots to il15 data

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Nathan Dwarshuis 2021-07-30 17:44:17 -04:00
parent 994df04db2
commit bca90222f3
3 changed files with 3990 additions and 101 deletions

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@ -3033,27 +3033,31 @@ many conditions, we hypothesized that \gls{il15} may be responsible for this,
and further that the unique \textit{cis/trans} activity of \gls{il15} may be and further that the unique \textit{cis/trans} activity of \gls{il15} may be
more active in the \gls{dms} system due to higher cell density. more active in the \gls{dms} system due to higher cell density.
% TODO actually add captions
% TODO add fold change and viability to these
% TODO maybe combine these
% TODO add some background into why IL15 is important and the proposed mechanism % TODO add some background into why IL15 is important and the proposed mechanism
\begin{figure*}[ht!] \begin{figure*}[ht!]
\begingroup \begingroup
\includegraphics{../figures/il15_blockade_1.png} \includegraphics{../figures/il15_blockade_1.png}
\phantomsubcaption\label{fig:il15_1_overview}
\phantomsubcaption\label{fig:il15_1_fc} \phantomsubcaption\label{fig:il15_1_fc}
\phantomsubcaption\label{fig:il15_1_viability} \phantomsubcaption\label{fig:il15_1_viability}
\phantomsubcaption\label{fig:il15_1_mem} \phantomsubcaption\label{fig:il15_1_mem}
\endgroup \endgroup
\caption[IL15 blocking I] \caption[IL15 blocking I]
{Blocking with IL15 does not lead to differences in memory or growth. {Blocking IL15Ra does not lead to differences in memory or growth.
\subcap{fig:il15_1_overview}{Experimental overview}
Longitudinal measurements of
\subcap{fig:il15_1_fc}{fold change} and
\subcap{fig:il15_1_viability}{viability} for blocked and unblocked
conditions expanded with either beads or \glspl{dms}.
\subcap{fig:il15_1_mem}{Flow cytometry markers for \gls{dms}-expanded T
cells at day 14 for blocked and unblocked groups.}.
} }
\label{fig:il15_1} \label{fig:il15_1}
\end{figure*} \end{figure*}
% TODO how did I determine how much to add? % TODO how did I determine how much to add?
% TODO how often was this added?
% TODO just gate these as normal because this looks sketchy % TODO just gate these as normal because this looks sketchy
We first tested this hypothesis by blocking \gls{il15r} with either a specific We first tested this hypothesis by blocking \gls{il15r} with either a specific
\gls{mab} or an \gls{igg} isotype control. We observed no difference in the \gls{mab} or an \gls{igg} isotype control. We observed no difference in the
@ -3070,13 +3074,21 @@ the markers, and by extension showing no difference in phenotype
\begingroup \begingroup
\includegraphics{../figures/il15_blockade_2.png} \includegraphics{../figures/il15_blockade_2.png}
\phantomsubcaption\label{fig:il15_2_overview}
\phantomsubcaption\label{fig:il15_2_fc} \phantomsubcaption\label{fig:il15_2_fc}
\phantomsubcaption\label{fig:il15_2_viability} \phantomsubcaption\label{fig:il15_2_viability}
\phantomsubcaption\label{fig:il15_2_mem} \phantomsubcaption\label{fig:il15_2_mem}
\endgroup \endgroup
\caption[IL15 blocking II] \caption[IL15 blocking II]
{Blocking with IL15 does not lead to differences in memory or growth. {Blocking soluble IL15 does not lead to differences in memory or growth.
\subcap{fig:il15_2_overview}{Experimental overview}
Longitudinal measurements of
\subcap{fig:il15_2_fc}{fold change} and
\subcap{fig:il15_2_viability}{viability} for blocked and unblocked
conditions expanded with \glspl{dms}.
\subcap{fig:il15_2_mem}{Flow cytometry markers for \gls{dms}-expanded T
cells at day 14 for blocked and unblocked groups.}.
} }
\label{fig:il15_2} \label{fig:il15_2}
\end{figure*} \end{figure*}