FIX typos
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@ -107,6 +107,7 @@
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\sisetup{per-mode=symbol,list-units=single}
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\sisetup{per-mode=symbol,list-units=single}
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\DeclareSIUnit\IU{IU}
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\DeclareSIUnit\IU{IU}
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\DeclareSIUnit\rpm{RPM}
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\DeclareSIUnit\rpm{RPM}
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\DeclareSIUnit\carrier{carrier}
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\DeclareSIUnit\dms{DMS}
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\DeclareSIUnit\dms{DMS}
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\DeclareSIUnit\cell{cells}
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\DeclareSIUnit\cell{cells}
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\DeclareSIUnit\ab{mAb}
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\DeclareSIUnit\ab{mAb}
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@ -1154,15 +1155,14 @@ higher, further showing that the decay of \gls{snb} is not a concern.
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We also investigated the reaction kinetics of all three coating steps.
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We also investigated the reaction kinetics of all three coating steps.
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To quantify the reaction kinetics of the biotin binding step, we reacted
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To quantify the reaction kinetics of the biotin binding step, we reacted
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multiple batches of microcarriers at \gls{rt} with
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multiple batches of \SI{20}{\mg\per\ml} microcarriers in \gls{pbs} at \gls{rt}
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\gls{20}{\mg\of{\carrier}\per\ml} with \gls{snb} in parallel and sacrificially
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with \gls{snb} in parallel and sacrificially analyzed each at varying timepoints
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analyzed each at varying timepoints using the \gls{haba} assay. This was
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using the \gls{haba} assay. This was performed at two different concentrations.
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performed at two different concentrations. We observed that for either
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We observed that for either concentration, the reaction was over in
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concentration, the reaction was over in \SIrange{20}{30}{\minute}
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\SIrange{20}{30}{\minute} (\cref{fig:dms_biotin_rxn_mass}). Furthermore, when
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(\cref{fig:dms_biotin_rxn_mass}). Furthermore, when put in terms of fraction of
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put in terms of fraction of input \gls{snb}, we observed that the curves are
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input \gls{snb}, we observed that the curves are almost identical
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almost identical (\cref{fig:dms_biotin_rxn_frac}). Given this, the reaction step
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(\cref{fig:dms_biotin_rxn_frac}). Given this, the reaction step for biotin
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for biotin attached was set to \SI{30}{\minute}.
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attached was set to \gls{30}{\minute}.
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% TODO these numbers might be totally incorrect
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% TODO these numbers might be totally incorrect
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Next, we quantified the amount of \gls{stp} reacted with the surface of the
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Next, we quantified the amount of \gls{stp} reacted with the surface of the
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