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ADD footnote explaining that I didn't do the nmr stuff

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Nathan Dwarshuis 2021-07-31 16:59:33 -04:00
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@ -2095,18 +2095,18 @@ Flow cytometry was performed analogously to \cref{sec:flow_cytometry}.
Cytokines were quantified via Luminex as described in Cytokines were quantified via Luminex as described in
\cref{sec:luminex_analysis}. \cref{sec:luminex_analysis}.
% TODO add a footnote saying I didn't do this
\subsection{NMR metabolomics} \subsection{NMR metabolomics}
Prior to analysis, samples were centrifuged at \SI{2990}{\gforce} for Prior to analysis, samples were centrifuged at \SI{2990}{\gforce} for
\SI{20}{\minute} at \SI{4}{\degreeCelsius} to clear any debris. 5 uL of 100/3 mM \SI{20}{\minute} at \SI{4}{\degreeCelsius} to clear any debris\footnote{all
DSS-D6 in deuterium oxide (Cambridge Isotope Laboratories) were added to 1.7 mm \gls{nmr} analysis was done by our collaborators at the University of
NMR tubes (Bruker BioSpin), followed by 45 uL of media from each sample that was Georgia}. 5 uL of 100/3 mM DSS-D6 in deuterium oxide (Cambridge Isotope
added and mixed, for a final volume of 50 uL in each tube. Samples were prepared Laboratories) were added to 1.7 mm NMR tubes (Bruker BioSpin), followed by 45 uL
on ice and in predetermined, randomized order. The remaining volume from each of media from each sample that was added and mixed, for a final volume of 50 uL
sample in the rack (approx. 4 uL) was combined to create an internal pool. This in each tube. Samples were prepared on ice and in predetermined, randomized
material was used for internal controls within each rack as well as metabolite order. The remaining volume from each sample in the rack (approx. 4 uL) was
annotation. combined to create an internal pool. This material was used for internal
controls within each rack as well as metabolite annotation.
\gls{nmr} spectra were collected on a Bruker Avance III HD spectrometer at 600 \gls{nmr} spectra were collected on a Bruker Avance III HD spectrometer at 600
MHz using a 5-mm TXI cryogenic probe and TopSpin software (Bruker BioSpin). MHz using a 5-mm TXI cryogenic probe and TopSpin software (Bruker BioSpin).