ADD footnote explaining that I didn't do the nmr stuff
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@ -2095,18 +2095,18 @@ Flow cytometry was performed analogously to \cref{sec:flow_cytometry}.
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Cytokines were quantified via Luminex as described in
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\cref{sec:luminex_analysis}.
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% TODO add a footnote saying I didn't do this
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\subsection{NMR metabolomics}
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Prior to analysis, samples were centrifuged at \SI{2990}{\gforce} for
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\SI{20}{\minute} at \SI{4}{\degreeCelsius} to clear any debris. 5 uL of 100/3 mM
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DSS-D6 in deuterium oxide (Cambridge Isotope Laboratories) were added to 1.7 mm
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NMR tubes (Bruker BioSpin), followed by 45 uL of media from each sample that was
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added and mixed, for a final volume of 50 uL in each tube. Samples were prepared
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on ice and in predetermined, randomized order. The remaining volume from each
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sample in the rack (approx. 4 uL) was combined to create an internal pool. This
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material was used for internal controls within each rack as well as metabolite
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annotation.
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\SI{20}{\minute} at \SI{4}{\degreeCelsius} to clear any debris\footnote{all
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\gls{nmr} analysis was done by our collaborators at the University of
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Georgia}. 5 uL of 100/3 mM DSS-D6 in deuterium oxide (Cambridge Isotope
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Laboratories) were added to 1.7 mm NMR tubes (Bruker BioSpin), followed by 45 uL
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of media from each sample that was added and mixed, for a final volume of 50 uL
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in each tube. Samples were prepared on ice and in predetermined, randomized
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order. The remaining volume from each sample in the rack (approx. 4 uL) was
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combined to create an internal pool. This material was used for internal
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controls within each rack as well as metabolite annotation.
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\gls{nmr} spectra were collected on a Bruker Avance III HD spectrometer at 600
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MHz using a 5-mm TXI cryogenic probe and TopSpin software (Bruker BioSpin).
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