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ADD footnote explaining that I didn't do the nmr stuff

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Nathan Dwarshuis 2021-07-31 16:59:33 -04:00
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tex/thesis.tex
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@ -2095,18 +2095,18 @@ Flow cytometry was performed analogously to \cref{sec:flow_cytometry}.
Cytokines were quantified via Luminex as described in
\cref{sec:luminex_analysis}.
% TODO add a footnote saying I didn't do this
\subsection{NMR metabolomics}
Prior to analysis, samples were centrifuged at \SI{2990}{\gforce} for
\SI{20}{\minute} at \SI{4}{\degreeCelsius} to clear any debris. 5 uL of 100/3 mM
DSS-D6 in deuterium oxide (Cambridge Isotope Laboratories) were added to 1.7 mm
NMR tubes (Bruker BioSpin), followed by 45 uL of media from each sample that was
added and mixed, for a final volume of 50 uL in each tube. Samples were prepared
on ice and in predetermined, randomized order. The remaining volume from each
sample in the rack (approx. 4 uL) was combined to create an internal pool. This
material was used for internal controls within each rack as well as metabolite
annotation.
\SI{20}{\minute} at \SI{4}{\degreeCelsius} to clear any debris\footnote{all
\gls{nmr} analysis was done by our collaborators at the University of
Georgia}. 5 uL of 100/3 mM DSS-D6 in deuterium oxide (Cambridge Isotope
Laboratories) were added to 1.7 mm NMR tubes (Bruker BioSpin), followed by 45 uL
of media from each sample that was added and mixed, for a final volume of 50 uL
in each tube. Samples were prepared on ice and in predetermined, randomized
order. The remaining volume from each sample in the rack (approx. 4 uL) was
combined to create an internal pool. This material was used for internal
controls within each rack as well as metabolite annotation.
\gls{nmr} spectra were collected on a Bruker Avance III HD spectrometer at 600
MHz using a 5-mm TXI cryogenic probe and TopSpin software (Bruker BioSpin).