ADD apoptosis figure
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@ -88,6 +88,8 @@
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\newacronym{colb}{COL-B}{collagenase B}
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\newacronym{cold}{COL-D}{collagenase D}
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\newacronym{tsne}{tSNE}{t-stochastic neighbor embedding}
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\newacronym{anv}{ANX-V}{Annexin-V}
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\newacronym{pi}{PI}{propidium iodide}
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%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
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% SI units for uber nerds
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@ -880,6 +882,8 @@ plates and incubated for another \SI{24}{\hour}. Cells and beads/\glspl{dms}
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were removed from the retronectin plates using vigorous pipetting and
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transferred to another 96 well plate wherein expansion continued.
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% METHOD for apoptosis quantification
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\subsection{statistical analysis}
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For 1-way \gls{anova} analysis with Tukey multiple comparisons test,
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@ -1066,7 +1070,28 @@ cells after \SI{14}{\day} were on the interior surface of the \glspl{dms}
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%, and this did not significantly change with initial seeding density (Supplemental Table 1).
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% FIGURE caspase/bcl2 figure
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\begin{figure*}[ht!]
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\begingroup
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\includegraphics{../figures/apoptosis.png}
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\phantomsubcaption\label{fig:apoptosis_annV}
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\phantomsubcaption\label{fig:apoptosis_cas}
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\phantomsubcaption\label{fig:apoptosis_bcl2}
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\endgroup
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\caption[Apoptosis Quantification for \glspl{dms}]
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{\glspl{dms} produce cells with lower apoptosis marker expression on average
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compared to bead.
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\subcap{fig:apoptosis_annV}{Quantification of apoptosis and necrosis by
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\gls{anv} and \gls{pi}.}
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\subcap{fig:apoptosis_cas}{Quantification of Caspase-3/7 expression using
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CellEvent dye.}
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\subcap{fig:apoptosis_bcl2}{Quantification of BCL-2 expression using
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\gls{elisa}. All statistical tests shown are two-tailed homoschodastic
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t-tests.}
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}
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\label{fig:dms_flowchart}
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\end{figure*}
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% TODO add this to the methods section
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% TODO double check the timing of this experiment (it might not be day 14)
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