ADD apoptosis figure

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Nathan Dwarshuis 2021-07-27 11:07:28 -04:00
parent 758b04c967
commit ed63f13797
2 changed files with 1385 additions and 1 deletions

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@ -88,6 +88,8 @@
\newacronym{colb}{COL-B}{collagenase B}
\newacronym{cold}{COL-D}{collagenase D}
\newacronym{tsne}{tSNE}{t-stochastic neighbor embedding}
\newacronym{anv}{ANX-V}{Annexin-V}
\newacronym{pi}{PI}{propidium iodide}
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
% SI units for uber nerds
@ -880,6 +882,8 @@ plates and incubated for another \SI{24}{\hour}. Cells and beads/\glspl{dms}
were removed from the retronectin plates using vigorous pipetting and
transferred to another 96 well plate wherein expansion continued.
% METHOD for apoptosis quantification
\subsection{statistical analysis}
For 1-way \gls{anova} analysis with Tukey multiple comparisons test,
@ -1066,7 +1070,28 @@ cells after \SI{14}{\day} were on the interior surface of the \glspl{dms}
%, and this did not significantly change with initial seeding density (Supplemental Table 1).
% FIGURE caspase/bcl2 figure
\begin{figure*}[ht!]
\begingroup
\includegraphics{../figures/apoptosis.png}
\phantomsubcaption\label{fig:apoptosis_annV}
\phantomsubcaption\label{fig:apoptosis_cas}
\phantomsubcaption\label{fig:apoptosis_bcl2}
\endgroup
\caption[Apoptosis Quantification for \glspl{dms}]
{\glspl{dms} produce cells with lower apoptosis marker expression on average
compared to bead.
\subcap{fig:apoptosis_annV}{Quantification of apoptosis and necrosis by
\gls{anv} and \gls{pi}.}
\subcap{fig:apoptosis_cas}{Quantification of Caspase-3/7 expression using
CellEvent dye.}
\subcap{fig:apoptosis_bcl2}{Quantification of BCL-2 expression using
\gls{elisa}. All statistical tests shown are two-tailed homoschodastic
t-tests.}
}
\label{fig:dms_flowchart}
\end{figure*}
% TODO add this to the methods section
% TODO double check the timing of this experiment (it might not be day 14)