ADD apoptosis figure

tex/thesis.tex

@@ -88,6 +88,8 @@
 \newacronym{colb}{COL-B}{collagenase B}
 \newacronym{cold}{COL-D}{collagenase D}
 \newacronym{tsne}{tSNE}{t-stochastic neighbor embedding}
+\newacronym{anv}{ANX-V}{Annexin-V}
+\newacronym{pi}{PI}{propidium iodide}
 
 %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%
 % SI units for uber nerds
@@ -880,6 +882,8 @@ plates and incubated for another \SI{24}{\hour}. Cells and beads/\glspl{dms}
 were removed from the retronectin plates using vigorous pipetting and
 transferred to another 96 well plate wherein expansion continued.
 
+% METHOD for apoptosis quantification
+
 \subsection{statistical analysis}
 
 For 1-way \gls{anova} analysis with Tukey multiple comparisons test,
@@ -1066,7 +1070,28 @@ cells after \SI{14}{\day} were on the interior surface of the \glspl{dms}
 
 %, and this did not significantly change with initial seeding density (Supplemental Table 1).
 
-% FIGURE caspase/bcl2 figure
+\begin{figure*}[ht!]
+  \begingroup
+
+  \includegraphics{../figures/apoptosis.png}
+  \phantomsubcaption\label{fig:apoptosis_annV}
+  \phantomsubcaption\label{fig:apoptosis_cas}
+  \phantomsubcaption\label{fig:apoptosis_bcl2}
+
+  \endgroup
+  \caption[Apoptosis Quantification for \glspl{dms}]
+  {\glspl{dms} produce cells with lower apoptosis marker expression on average
+    compared to bead.
+    \subcap{fig:apoptosis_annV}{Quantification of apoptosis and necrosis by
+      \gls{anv} and \gls{pi}.}
+    \subcap{fig:apoptosis_cas}{Quantification of Caspase-3/7 expression using
+      CellEvent dye.}
+    \subcap{fig:apoptosis_bcl2}{Quantification of BCL-2 expression using
+      \gls{elisa}. All statistical tests shown are two-tailed homoschodastic
+      t-tests.}
+  }
+  \label{fig:dms_flowchart}
+\end{figure*}
 
 % TODO add this to the methods section
 % TODO double check the timing of this experiment (it might not be day 14)