ADD footnote explaining that these aren't the donors you are looking for

This commit is contained in:
Nathan Dwarshuis 2021-09-06 23:53:14 -04:00
parent 30158fed3e
commit f8484943ff
1 changed files with 15 additions and 16 deletions

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@ -2321,8 +2321,6 @@ cells to a greater degree than beads \cref{fig:dms_exp_cd4}. The trends held
true when observing the CD4+ and CD8+ fractions of the naïve/memory subset
(\ptmem{}) (\cref{fig:dms_exp_mem4,fig:dms_exp_mem8}).
% FIGURE this figure was not produced with the same donors as the figure above,
% which is really confusing
\begin{figure*}[ht!]
\begingroup
@ -2345,20 +2343,21 @@ We also observed that, at least with the donors and conditions tested in these
experiments\footnote{these results were not always consistent, see the
metaanalysis at the end of this aim for an in-depth quantification of this
observation} that the fraction of \ptmem{} and \pth{} T cells was higher in
the \gls{dms} groups compared to the bead groups (\cref{fig:dms_phenotype}).
This result was seen for multiple donors. We should note that in the case of
\pthp{}, the donors we used had an initial \pthp{} that was much higher (healthy
donors generally have a CD4:CD8 ratio of 2:1), so the proper interpretation of
this is that the \pthp{} decreases less over the culture period with the
\gls{dms} platform as opposed to the beads (or alternatively, the \gls{dms} has
less preferential expansion for CD8 T cells). We cannot say the same about
the \ptmemp{} since we did not have the initial data for this phenotype;
however (although it should be the vast majority of cells given that
cryopreserved T cells from a healthy donor should generally be composed of
circulated memory and naive T cells). Taken together, these data indicate the
\gls{dms} platform has the capacity to expand higher numbers and percentages of
highly potent \ptmem{} and \pth{} T cells compared to state-of-the-art bead
technology.
the \gls{dms} groups compared to the bead groups
(\cref{fig:dms_phenotype})\footnote{these where not the same donors as used for
\cref{fig:dms_exp}}. This result was seen for multiple donors. We should note
that in the case of \pthp{}, the donors we used had an initial \pthp{} that was
much higher (healthy donors generally have a CD4:CD8 ratio of 2:1), so the
proper interpretation of this is that the \pthp{} decreases less over the
culture period with the \gls{dms} platform as opposed to the beads (or
alternatively, the \gls{dms} has less preferential expansion for CD8 T cells).
We cannot say the same about the \ptmemp{} since we did not have the initial
data for this phenotype; however (although it should be the vast majority of
cells given that cryopreserved T cells from a healthy donor should generally be
composed of circulated memory and naive T cells). Taken together, these data
indicate the \gls{dms} platform has the capacity to expand higher numbers and
percentages of highly potent \ptmem{} and \pth{} T cells compared to
state-of-the-art bead technology.
\subsection*{DMSs can be used to produce functional CAR T cells}