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ADD discussion to aim 3 mouse 1

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Nathan Dwarshuis 2021-07-27 13:05:01 -04:00
parent 3227c00a8a
commit c610ddb40e
1 changed files with 18 additions and 2 deletions
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tex/thesis.tex
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@ -157,6 +157,7 @@
\newcommand{\ptmemh}{\pth\ptmem} \newcommand{\ptmemh}{\pth\ptmem}
\newcommand{\ptmemk}{\ptk\ptmem} \newcommand{\ptmemk}{\ptk\ptmem}
\newcommand{\dpthp}{$\Updelta$\pthp{}} \newcommand{\dpthp}{$\Updelta$\pthp{}}
\newcommand{\carp}{\gls{car}+~\si{\percent}}
\newcommand{\catnum}[2]{(#1, #2)} \newcommand{\catnum}[2]{(#1, #2)}
\newcommand{\product}[3]{#1 \catnum{#2}{#3}} \newcommand{\product}[3]{#1 \catnum{#2}{#3}}
@ -2072,8 +2073,7 @@ cells are effective at lower doses.
\subcap{fig:mouse_timecourse_qc_growth}{Fold change of T cells (each \subcap{fig:mouse_timecourse_qc_growth}{Fold change of T cells (each
timepoint only includes the runs that were harvested at day 14).} timepoint only includes the runs that were harvested at day 14).}
Fractions of T cell subtypes in the day 14 product including Fractions of T cell subtypes in the day 14 product including
% TODO refactor CAR+ percent into nice macro \subcap{fig:mouse_timecourse_qc_car}{\carp{}},
\subcap{fig:mouse_timecourse_qc_car}{\gls{car}+~\si{\percent}},
\subcap{fig:mouse_timecourse_qc_cd4}{\pthp{}}, and \subcap{fig:mouse_timecourse_qc_cd4}{\pthp{}}, and
\subcap{fig:mouse_timecourse_qc_mem}{\ptmemp{}}. \subcap{fig:mouse_timecourse_qc_mem}{\ptmemp{}}.
} }
@ -2103,6 +2103,22 @@ cells are effective at lower doses.
\section{discussion} \section{discussion}
When we tested bead and DMS expanded \gls{car} T cells, we also found that the
\gls{dms} expanded CAR-T cells outperformed bead groups in prolonging survival
of Nalm-6 tumor challenged (intravenously injected) \gls{nsg} mice. DMS expanded
CAR-T cells were very effective in clearing tumor cells as early as 7 days post
CAR-T injection even at low total T cell dose compared to the bead groups where
tumor burden was higher than DMS groups across all the total T cell doses tested
here. More interestingly, when only CAR-expressing T cell doses between bead and
DMS groups were compared, DMS group had significantly higher survival effects
over similar or higher CAR expression T cell doses from bead group. All these
results suggest that the higher proportion of memory T cells in DMS groups
(compared to bead group) resulted in highly effective CAR-T cells that can
efficiently kill tumor cells as recently reported in
literature\cite{Fraietta2018, Sommermeyer2015}.
% DISCUSSION 2nd mouse model
\chapter{conclusions and future work}\label{conclusions} \chapter{conclusions and future work}\label{conclusions}
\section{conclusions} \section{conclusions}
\section{future work} \section{future work}